{"title":"Engineering branching morphogenesis using cell communication","authors":"Chloé D. Devillard, Christophe A. Marquette","doi":"10.1016/j.bprint.2023.e00261","DOIUrl":null,"url":null,"abstract":"<div><p><span>Branching morphogenesis<span>, a specialized part of morphogenesis<span><span>, leads to the formation of microstructures (tubes, canals, and glands), source of the active organ functions. The dynamic mechanisms involved are appearance/disappearance of biomolecules </span>morphogens<span> gradients. In the context of angiogenesis, growth factors allow the initiation, regulation, and remodeling of blood vessels. In the particular case of micro-vascularization, it seems essential to reproduce and study the interaction of </span></span></span></span>endothelial cells with their environment but also with other cellular components, including fibroblasts.</p><p>To bring understanding here, we developed an angiogenesis 3D bioprinted (microextrusion bioprinting) model based on a proliferative bioink (7.5% (w/v) gelatin, 0.5% (w/v) alginate<span>, 2% (w/v) fibrinogen) populated with fibroblasts and HUVECs. We demonstrated that we were able to recapitulate branching angiogenesis, producing organized microvascularization tissue in 7 days only.</span></p><p><span>We clearly demonstrated that a bidirectional communication was at stake between the two cell types, evidenced only when both types were culture in a 3D environment. Proteomic<span> results (multiplexed ELISA) consolidated the understanding of this phenomenon, with 11 angiogenic proteins identified in the co-culture supernatant. They were identified as inducers of vasculogenesis and angiogenesis. Through matrix composition and cell organization study, we were able to demonstrate that tissue remodeling, </span></span>extracellular matrix production (type I collagen), phenotype modification (pericytes) were taking place in our branching morphogenesis model.</p><p><span>Thanks to this breakthrough scientific advance in the field of regenerative medicine, we can imagine the </span>biofabrication of functional tissues and organs models in the coming decades.</p></div>","PeriodicalId":37770,"journal":{"name":"Bioprinting","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Bioprinting","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2405886623000040","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"Computer Science","Score":null,"Total":0}
引用次数: 0
Abstract
Branching morphogenesis, a specialized part of morphogenesis, leads to the formation of microstructures (tubes, canals, and glands), source of the active organ functions. The dynamic mechanisms involved are appearance/disappearance of biomolecules morphogens gradients. In the context of angiogenesis, growth factors allow the initiation, regulation, and remodeling of blood vessels. In the particular case of micro-vascularization, it seems essential to reproduce and study the interaction of endothelial cells with their environment but also with other cellular components, including fibroblasts.
To bring understanding here, we developed an angiogenesis 3D bioprinted (microextrusion bioprinting) model based on a proliferative bioink (7.5% (w/v) gelatin, 0.5% (w/v) alginate, 2% (w/v) fibrinogen) populated with fibroblasts and HUVECs. We demonstrated that we were able to recapitulate branching angiogenesis, producing organized microvascularization tissue in 7 days only.
We clearly demonstrated that a bidirectional communication was at stake between the two cell types, evidenced only when both types were culture in a 3D environment. Proteomic results (multiplexed ELISA) consolidated the understanding of this phenomenon, with 11 angiogenic proteins identified in the co-culture supernatant. They were identified as inducers of vasculogenesis and angiogenesis. Through matrix composition and cell organization study, we were able to demonstrate that tissue remodeling, extracellular matrix production (type I collagen), phenotype modification (pericytes) were taking place in our branching morphogenesis model.
Thanks to this breakthrough scientific advance in the field of regenerative medicine, we can imagine the biofabrication of functional tissues and organs models in the coming decades.
期刊介绍:
Bioprinting is a broad-spectrum, multidisciplinary journal that covers all aspects of 3D fabrication technology involving biological tissues, organs and cells for medical and biotechnology applications. Topics covered include nanomaterials, biomaterials, scaffolds, 3D printing technology, imaging and CAD/CAM software and hardware, post-printing bioreactor maturation, cell and biological factor patterning, biofabrication, tissue engineering and other applications of 3D bioprinting technology. Bioprinting publishes research reports describing novel results with high clinical significance in all areas of 3D bioprinting research. Bioprinting issues contain a wide variety of review and analysis articles covering topics relevant to 3D bioprinting ranging from basic biological, material and technical advances to pre-clinical and clinical applications of 3D bioprinting.