Anti-inflammatory, Analgesic, and Cytotoxic Effects of The Phytexponent: A Polyherbal Formulation

IF 3.3 Q1 INTEGRATIVE & COMPLEMENTARY MEDICINE
Halvince O Odira, Simon O Mitema, I. Mapenay, G. Moriasi
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引用次数: 5

Abstract

The Phytexponent is used to treat pain and inflammation in complementary and alternative medicine practices; however, empirical data supporting its pharmacological efficacy and safety is scanty, hence the present study. We used the carrageenan-induced paw oedema and the acetic acid-induced writhing techniques to determine the anti-inflammatory and analgesic efficacies, respectively, of the Phytexponent in Swiss albino mice models. The 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay technique was used to investigate the in vitro cytotoxic effects of the Phytexponent in the Vero E6 cell line. The Phytexponent exerted significant (P < .05) anti-inflammatory effects in the carrageenan-induced paw oedema mouse model in a dose- and time-dependent manner, with significantly higher efficacy at 250 mg/Kg BW, than indomethacin (4 mg/Kg BW), in the first, second, and third hour (P < .05). Besides, the Phytexponent significantly reduced the acetic acid-induced writhing frequency in mice (P < .05), in a dose-dependent manner, depicting its analgesic efficacy. Notably, the Phytexponent (at doses: 125 mg/Kg BW and 250 mg/Kg BW) exhibited significantly higher analgesic efficacy than the Indomethacin (P<.05). Moreover, the Phytexponent was not cytotoxic to Vero E6 cells (CC50 >1000 µg/ml) compared to cyclophosphamide (CC50 = 2.48 µg/ml). Thus, the Phytexponent has significant in vivo anti-inflammatory and analgesic efficacy in mice models and is not cytotoxic to Vero E6 cell line, depicting its therapeutic potential upon further empirical investigation.
植物指数的抗炎、镇痛和细胞毒性作用:一种多草药制剂
Phytexponent用于治疗补充和替代医学实践中的疼痛和炎症;然而,支持其药理有效性和安全性的经验数据很少,因此本研究。采用角叉菜胶诱导足跖水肿和醋酸诱导扭体技术分别测定Phytexponent对瑞士白化小鼠模型的抗炎和镇痛作用。采用3-(4,5 -二甲基噻唑-2-基)- 2,5 -二苯基溴化四唑(MTT)法研究了Phytexponent对Vero E6细胞株的体外细胞毒作用。与环磷酰胺(CC50 = 2.48µg/ml)相比,Phytexponent (P = 1000µg/ml)具有显著性(P = 1000µg/ml)。因此,在小鼠模型中,Phytexponent具有显著的体内抗炎和镇痛作用,并且对Vero E6细胞系没有细胞毒性,进一步的实证研究表明其具有治疗潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Journal of Evidence-based Integrative Medicine
Journal of Evidence-based Integrative Medicine INTEGRATIVE & COMPLEMENTARY MEDICINE-
CiteScore
5.90
自引率
0.00%
发文量
43
审稿时长
15 weeks
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