Structural comparison of different isolated eucalyptus lignins and analysis of their interaction mechanism with bovine serum albumin solution under QCM-D

IF 2.2 3区农林科学 Q2 FORESTRY

Holzforschung Pub Date : 2023-04-17 DOI:10.1515/hf-2022-0170

Haonan Zhang, Qi Liu, Yanchen Zhu, Zhang Feng, Hao Ren, H. Zhai

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引用次数: 0

Abstract

Abstract In this study, representative lignin samples differing in hydroxyl content, molecular weight, linkage composition and lignin units ratio were prepared from eucalyptus wood by different isolation means and modification means: milled wood lignin (MWL), kraft lignin classified at different pH (KL), lignocresol (LC), lignoresorcinol (LR), lignopyrogallol (LP) as well as lignocresol secondary functional switched samples (LC 2ndderiv). The structure of various lignins was characterized in detail by Fourier transform infrared (FT-IR) spectroscopy, gel permeation chromatography (GPC) and nuclear magnetic resonance (NMR). In order to eliminate the interference of other factors, bovine serum albumin (BSA) with relatively simple protein structure and different kinds of lignin and their derivatives were selected as the research objects. The adsorption/desorption behavior and mechanism between lignin samples and BSA were studied by quartz crystal microbalance with dissipation monitoring (QCM-D). The results showed that the content of phenolic hydroxyl was the main factor affecting the interaction of lignin and BSA, but in addition, the molecular size, linkages composition and the types of lignin units also had important effects on its adsorption behavior. The research will provide theoretical guidance and all-round reliable basic data for the interaction of lignin on lignocellulose hydrolysis enzymes in industrial application.

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不同分离桉树木质素的结构比较及其与牛血清白蛋白溶液相互作用机制的QCM-D分析

摘要本研究以桉树为原料，采用不同的分离和改性方法制备了羟基含量、分子量、连接组成和木质素单元比不同的代表性木质素样品：磨木木质素（MWL）、不同pH下分类的硫酸盐木质素（KL）、木质甲酚（LC）、木质间苯二酚（LR），木质邻苯三酚（LP）以及木质甲酚二级功能切换样品（LC 2ndderiv）。通过傅立叶变换红外光谱、凝胶渗透色谱和核磁共振对各种木质素的结构进行了详细表征。为了消除其他因素的干扰，选择蛋白质结构相对简单的牛血清白蛋白（BSA）和不同种类的木质素及其衍生物作为研究对象。采用耗散监测石英晶体微天平（QCM-D）研究了木质素样品与BSA的吸附/解吸行为及其机理。结果表明，酚羟基含量是影响木质素与BSA相互作用的主要因素，但分子大小、键组成和木质素单元类型对其吸附行为也有重要影响。该研究将为木质素与木质纤维素水解酶的相互作用在工业应用中提供理论指导和全方位可靠的基础数据。

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来源期刊

Holzforschung 工程技术-材料科学：纸与木材

CiteScore

4.60

自引率

4.20%

发文量

审稿时长

3.3 months

期刊介绍： Holzforschung is an international scholarly journal that publishes cutting-edge research on the biology, chemistry, physics and technology of wood and wood components. High quality papers about biotechnology and tree genetics are also welcome. Rated year after year as one of the top scientific journals in the category of Pulp and Paper (ISI Journal Citation Index), Holzforschung represents innovative, high quality basic and applied research. The German title reflects the journal''s origins in a long scientific tradition, but all articles are published in English to stimulate and promote cooperation between experts all over the world. Ahead-of-print publishing ensures fastest possible knowledge transfer.