Comparison of Aminoglycoside Antibiotics and Cobalt Chloride for Ablation of the Lateral Line System in Giant Danios

IF 2.2 4区 生物学 Q2 BIOLOGY
P. J. Mekdara, S. Tirmizi, M. A. B. Schwalbe, E. Tytell
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引用次数: 2

Abstract

Synopsis The mechanoreceptive lateral line system in fish is composed of neuromasts containing hair cells, which can be temporarily ablated by aminoglycoside antibiotics and heavy metal ions. These chemicals have been used for some time in studies exploring the functional role of the lateral line system in many fish species. However, little information on the relative effectiveness and rate of action of these chemicals for ablation is available. In particular, aminoglycoside antibiotics are thought to affect canal neuromasts, which sit in bony or trunk canals, differently from superficial neuromasts, which sit directly on the skin. This assumed ablation pattern has not been fully quantified for commonly used lateral line ablation agents. This study provides a detailed characterization of the effects of two aminoglycoside antibiotics, streptomycin sulfate and neomycin sulfate, and a heavy metal salt, cobalt (II) chloride hexahydrate (CoCl2), on the ablation of hair cells in canal and superficial neuromasts in the giant danio (Devario aequipinnatus) lateral line system, as a model for adult teleost fishes. We also quantified the regeneration of hair cells after ablation using CoCl2 and gentamycin sulfate to verify the time course to full recovery, and whether the ablation method affects the recovery time. Using a fluorescence stain, 4-Di-2-ASP, we verified the effectiveness of each chemical by counting the number of fluorescing canal and superficial neuromasts present throughout the time course of ablation and regeneration of hair cells. We found that streptomycin and neomycin were comparably effective at ablating all neuromasts in less than 12 h using a 250 μM dosage and in less than 8 h using a 500 μM dosage. The 500 μM dosage of either streptomycin or neomycin can ablate hair cells in superficial neuromasts within 2–4 h, while leaving those in canal neuromasts mostly intact. CoCl2 (0.1 mM) worked the fastest, ablating all of the hair cells in less than 6 h. Complete regeneration of the neuromasts in the lateral line system took 7 days regardless of chemicals used to ablate the hair cells. This study adds to the growing knowledge in hearing research about how effective specific chemicals are at ablating hair cells in the acoustic system of vertebrates.
氨基糖苷类抗生素与氯化钴消融巨蟒侧线系统的比较
鱼的机械感受侧线系统是由含有毛细胞的神经突组成的,它可以被氨基糖苷类抗生素和重金属离子暂时消融。这些化学物质已经被用于研究许多鱼类的侧线系统的功能作用。然而,关于这些化学物质消融的相对有效性和作用率的信息很少。特别是,氨基糖苷类抗生素被认为会影响位于骨或干管中的管状神经根,与直接位于皮肤上的浅表神经根不同。对于常用的侧线消融剂,这种假定的消融模式尚未完全量化。本研究详细描述了两种氨基糖苷类抗生素,硫酸链霉素和硫酸新霉素,以及一种重金属盐,六水氯化钴(CoCl2),对巨鱼(Devario aequipinnatus)侧线系统毛细胞和浅表神经鞘消融的影响,作为成年硬骨鱼的模型。我们还量化了CoCl2和硫酸庆大霉素消融后毛细胞的再生情况,以验证完全恢复的时间过程,以及消融方法是否影响恢复时间。使用荧光染色剂4-Di-2-ASP,我们通过计算在毛细胞消融和再生的整个时间过程中存在的荧光管和浅表神经鞘的数量来验证每种化学物质的有效性。我们发现链霉素和新霉素在250 μM剂量下和500 μM剂量下分别在不到12小时和不到8小时内有效消融所有神经鞘。500 μM剂量的链霉素或新霉素均能在2 ~ 4 h内使浅表神经鞘的毛细胞消融,而管状神经鞘的毛细胞基本完好。CoCl2 (0.1 mM)作用最快,在不到6小时内消融所有毛细胞。无论使用何种化学物质消融毛细胞,侧线系统神经突的完全再生都需要7天。这项研究增加了听力研究中关于特定化学物质如何有效地消融脊椎动物声学系统中的毛细胞的知识。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
3.70
自引率
6.70%
发文量
48
审稿时长
20 weeks
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