The Influence of Pantogam and Atomoxetine on Attention Stability and Distribution of Dopamine D2 and GABAB Receptors in the Attention Deficit Mouse Model

IF 0.6 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Biochemistry (Moscow), Supplement Series B: Biomedical Chemistry Pub Date : 2022-02-14 DOI:10.1134/S1990750822010048

G. I. Kovalev, N. A. Sukhorukova, E. V. Vasileva, E. A. Kondrakhin, R. M. Salimov

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引用次数: 0

Abstract—

The closed enriched cross maze test was employed as a novel experimental model of the attention deficit disorder (ADD) for evaluation of the behavioral and neurochemical effects of the nootropic drug pantogam (100 mg/kg, intraperitoneally) and atomoxetine hydrochloride (3 mg/kg, intraperitoneally) administered subchronically to CD-1 outbred mice. Two subpopulations of rodents spontaneously diverging in attention to enriched compartments (ED-Low and ED-High), were estimated on the basis of time spent by the mice in the empty or enriched compartments. The ED-Low and ED-High mice did not differ in parameters associated with anxiety, exploratory efficacy, and locomotor activity. Subchronic administration of both drugs in selected doses produced a corrective effect on animal behavior manifested as selective increase in the ED-ratio values in the ED-Low subpopulation (p < 0.05). The radioligand binding assays revealed differences in the distribution of dopamine D₂ and GABA_B receptors (B_max) in prefrontal cortex of control ED-Low and ED-High mice. In prefrontal cortex of ED-Low mice treatment with atomoxetine produced a decrease in the B_max values of D₂ receptors by 14%, while pantogam decreased the B_max values of D₂ receptors by 22% (p < 0.05) and increased the B_max values for GABA_B receptor binding by 44%. Thus, subchronic administration of pantogam had a selective corrective effect on the behavior parameters and the density of the studied receptor subtypes in animals having had severe attention deficit in the test.

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泮托甘和托莫西汀对注意缺陷小鼠注意稳定性及多巴胺D2和GABAB受体分布的影响

摘要采用封闭富集交叉迷宫实验作为注意缺陷障碍(ADD)的新实验模型，评价促智药物pantogam (100 mg/kg，腹腔)和盐酸托莫西汀(3 mg/kg，腹腔)亚慢性给药CD-1远交种小鼠的行为和神经化学作用。根据小鼠在空室或高室中花费的时间，估计了两种啮齿类动物亚群在注意力上自发分化到富集室(ED-Low和ED-High)。ED-Low和ED-High小鼠在焦虑、探索效能和运动活动相关参数上没有差异。两种药物的亚慢性剂量均对动物行为产生矫正作用，表现为ED-Low亚群ED-ratio值的选择性增加(p <0.05)。放射配体结合实验显示，ED-Low和ED-High小鼠前额皮质多巴胺D2和GABAB受体(Bmax)的分布存在差异。在ED-Low小鼠的前额皮质中，托莫西汀使D2受体的Bmax值降低了14%，而pantogam使D2受体的Bmax值降低了22% (p <0.05)，使GABAB受体结合Bmax值提高44%。因此，亚慢性给药pantogam对实验中有严重注意力缺陷的动物的行为参数和所研究的受体亚型的密度有选择性的纠正作用。

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来源期刊

Biochemistry (Moscow), Supplement Series B: Biomedical Chemistry BIOCHEMISTRY & MOLECULAR BIOLOGY-

CiteScore

1.10

自引率

0.00%

发文量

期刊介绍： Biochemistry (Moscow), Supplement Series B: Biomedical Chemistry covers all major aspects of biomedical chemistry and related areas, including proteomics and molecular biology of (patho)physiological processes, biochemistry, neurochemistry, immunochemistry and clinical chemistry, bioinformatics, gene therapy, drug design and delivery, biochemical pharmacology, introduction and advertisement of new (biochemical) methods into experimental and clinical medicine. The journal also publishes review articles. All issues of the journal usually contain solicited reviews.