Exploitation of the antibacterial, antibiofilm and antioxidant activities of Salvadora Persica (Miswak) extract

IF 20.2 Q1 MATERIALS SCIENCE, PAPER & WOOD
Gamal M. El-Sherbiny, Ahmed Mohamed Gazelly, Mohammed H. Sharaf, Saad A. Moghannemm, Shehata M. E, Mahmoud K.A. Ismail, Ahmad S. El-Hawary
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引用次数: 4

Abstract

The Salvadora persica (S. persica) L. chewing stick, usually known as miswak, is still being employed as an oral hygiene agent for plaque and gingivitis prevention. This study aims to assess the antibacterial, antibiofilm, antioxidant, and phytochemical profile of S. persica extract. The S. persica was purchased from a local market, grinded and extracted with petroleum ether. The disk diffusion, microdilution, and micro-plate assays were performed to evaluate the antibacterial and antibiofilm activities of the prepared extract at different concentrations against β-lactam resistance Streptococcus species. Free radical scavenging 2,2-diphenyl-1-picrylhydrazyl (DPPH) and stable radical cationic chromophore, 2,2-azinobis-(3-ethylbenzothiazoline-6-sulfonate) (ABTS) methods were used to determine their antioxidant activity. Chromatographic and spectrometric analyses were performed using gas chromatography-mass (GC-MS) spectrometry. The minimum inhibitory concentration (MIC) of S. persica extract against β-lactam resistance Streptococcus species ranged from 6.25 to 12.5 mg/mL. The maximum suppression of biofilm formation by S. persica extract was observed at MIC with a percentage of 68.66%, against Streptococcus oralis. The S. persica extract exhibited antioxidant activity with IC50 of 20 µg/mL and 35 µg/mL from DPPH and ABTS, respectively. The phytochemical characterization showed the presence of 22 compounds with major compounds; benzyl isothiocyanate (36.21%) and n-hexadecanoic acid (27.62%). The S. persica extract exhibited antibacterial activity against β-lactam resistant Streptococcus species, showing a promising natural alternative that could be a treatment option.

萨尔瓦多Persica(Miswak)提取物的抗菌、抗菌膜和抗氧化活性的开发
番石榴(S. persica) L.咀嚼棒,通常被称为miswak,仍然被用作预防牙菌斑和牙龈炎的口腔卫生剂。本研究旨在研究山核桃提取物的抗菌、抗生物膜、抗氧化和植物化学特性。桃香是从当地市场购买的,用石油醚研磨和提取。采用圆盘扩散法、微稀释法和微平板法评价不同浓度提取物对β-内酰胺耐药链球菌的抑菌活性和抗生物膜活性。以自由基清除能力2,2-二苯基-1-吡啶肼基(DPPH)和稳定自由基阳离子发色团2,2-氮唑-(3-乙基苯并噻唑-6-磺酸盐)(ABTS)测定其抗氧化活性。色谱和光谱分析采用气相色谱-质谱(GC-MS)法。桃金酸提取物对β-内酰胺耐药链球菌的最小抑制浓度(MIC)为6.25 ~ 12.5 mg/mL。桃红提取物对口腔链球菌生物膜形成的抑制作用在MIC时最大,抑制率为68.66%。DPPH和ABTS提取物的IC50分别为20µg/mL和35µg/mL。植物化学鉴定结果表明,其中有22个化合物含主要化合物;异硫氰酸苄酯(36.21%)和正十六酸(27.62%)。枸杞提取物对β-内酰胺耐药链球菌具有抗菌活性,是一种有前景的天然替代治疗选择。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Journal of Bioresources and Bioproducts
Journal of Bioresources and Bioproducts Agricultural and Biological Sciences-Forestry
CiteScore
39.30
自引率
0.00%
发文量
38
审稿时长
12 weeks
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