The Influence of Drying Method on the Anthocyanin Character of Duwet Fruit Qualitatively and Quantitatively

L. H. Hidayat, D. G. Wirasisya, H. Muliasari
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Abstract

The fruit of duwet (Syzygium cumini L.) has anthocyanin compounds with some pharmacological effects.  Several factors, such as the drying method, determined anthocyanin levels.  This study aimed to compare the drying method's effect on anthocyanins' qualitative and quantitative characteristics in duwet fruit.  The drying methods tested were (1) fresh fruit (without drying), (2) freeze drying, and (3) sun drying.  The sample was macerated with 96% alcohol and 3% citric acid (6:1) and concentrated using a rotary evaporator.  The extracts were then qualitatively analyzed with color and TLC tests.  Anthocyanin levels were analyzed by the UV-Vis spectrophotometer.  The red and green colors of each sample indicated the presence of anthocyanins.  Rf values of 0.25; 0.375; 0.5 indicated anthocyanin type, which was cyanidin 3-ramnosil glucoside 5-glucoside, cyanidin 3-glucoside, and petunidin 3,7 diglucoside.  The average anthocyanin content in the freeze-drying and sun-drying method were successively 305.406 mg.l-1 and 99.0244 mg.l-1.  The freeze-drying method showed stronger color intensity and higher anthocyanin levels (p<0.05) than sun drying.
干燥方法对杜杜果花色苷特性的定性和定量影响
duwet(Syzygium cumini L.)果实中含有花青素化合物,具有一定的药理作用。几个因素,如干燥方法,决定了花青素的水平。本研究旨在比较干燥方法对羽绒果实花青素定性和定量特性的影响。测试的干燥方法为(1)新鲜水果(不干燥)、(2)冷冻干燥和(3)阳光干燥。样品用96%的酒精和3%的柠檬酸(6:1)浸渍,并使用旋转蒸发器浓缩。然后用颜色和薄层色谱法对提取物进行定性分析。用紫外-可见分光光度计分析花青素含量。每个样品的红色和绿色表明花青素的存在。Rf值为0.25;0.375;0.5表示花青素类型,为矢车菊素3-葡萄糖苷-5-葡萄糖苷、矢车菊苷-3-葡萄糖苷和紫藤素3,7-二葡萄糖苷。冷冻干燥法和日光干燥法的平均花青素含量分别为305.406mg.l-1和99.0244mg.l-1。冷冻干燥法比日光干燥法表现出更强的颜色强度和更高的花青素水平(p<0.05)。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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