Antipathy of Trichoderma against Sclerotium rolfsii Sacc.: Evaluation of Cell Wall-Degrading Enzymatic Activities and Molecular Diversity Analysis of Antagonists

IF 1.2 Q2 Biochemistry, Genetics and Molecular Biology

Journal of Molecular Microbiology and Biotechnology Pub Date : 2017-01-13 DOI:10.1159/000452997

D. Hirpara, H. Gajera, H. Z. Hirpara, B. Golakiya

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引用次数: 26

Abstract

The fungus Trichoderma is a teleomorph of the Hypocrea genus and associated with biological control of plant diseases. The microscopic, biochemical, and molecular characterization of Trichoderma was carried out and evaluated for in vitro antagonistic activity against the fungal pathogen Sclerotium rolfsii causing stem rot disease in groundnut. In total, 11 isolates of Trichoderma were examined for antagonism at 6 and 12 days after inoculation (DAI). Out of 11, T. virens NBAII Tvs12 evidenced the highest (87.91%) growth inhibition of the test pathogen followed by T. koningii MTCC 796 (67.03%), T. viride NBAII Tv23 (63.74%), and T. harzianum NBAII Th1 (60.44%). Strong mycoparasitism was observed in the best antagonist Tvs12 strain during 6-12 DAI. The specific activity of cell wall-degrading enzymes - chitinase and β-1,3-glucanase - was positively correlated with growth inhibition of the test pathogen. In total, 18 simple sequence repeat (SSR) polymorphisms were reported to amplify 202 alleles across 11 Trichoderma isolates. The average polymorphism information content for SSR markers was found to be 0.80. The best antagonist Tvs 12 was identified with 7 unique SSR alleles amplified by 5 SSR markers. Clustering patterns of 11 Trichoderma strains showed the best antagonist T. virens NBAII Tvs 12 outgrouped with a minimum 3% similarity from the rest of Trichoderma.

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木霉对大豆硬核病的拮抗作用：细胞壁降解酶活性评价及拮抗剂分子多样性分析

木霉是Hypocrea属的硬骨菌，与植物病害的生物防治有关。对木霉的显微、生化和分子特征进行了表征，并评价了木霉对引起花生茎腐病的真菌病原体硬柄菌的体外拮抗活性。总共在接种后6天和12天检测了11个木霉分离株的拮抗作用（DAI）。在11个菌株中，T.virens NBAII-Tvs12对试验病原体的生长抑制作用最高（87.91%），其次是T.koningii MTCC 796（67.03%）、T.viride NBAII-Tv23（63.74%）和T.harzianum NBAII-Th1（60.44%）。细胞壁降解酶几丁质酶和β-1,3-葡聚糖酶的比活性与试验病原体的生长抑制呈正相关。据报道，共有18个简单序列重复序列（SSR）多态性扩增了11个木霉分离株的202个等位基因。SSR标记的平均多态性信息含量为0.80。用5个SSR标记扩增出7个独特的SSR等位基因，鉴定出最佳拮抗剂Tvs12。11株木霉的聚类模式显示，最佳拮抗剂T.virens NBAII Tvs 12与其他木霉的相似性最低为3%。

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来源期刊

Journal of Molecular Microbiology and Biotechnology 生物-生物工程与应用微生物

CiteScore

3.90

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： We are entering a new and exciting era of microbiological study and application. Recent advances in the now established disciplines of genomics, proteomics and bioinformatics, together with extensive cooperation between academic and industrial concerns have brought about an integration of basic and applied microbiology as never before.