Inhibitory effects of methanol extracts from Fallopia japonica and F. × bohemica rhizomes and selected phenolic compounds on radish germination and root growth

Abstract

Allelopathic plants release secondary compounds into the soil that then suppress the growth of nearby plants. Allelopathy has been shown for the invasive Japanese knotweed (Fallopia japonica) and Bohemian knotweed (F. × bohemica). The aggressive and dominant invaders represent a serious threat to the local plant communities outside their native range. Here, we analysed the phenols in the knotweed rhizomes using nuclear magnetic resonance. We also evaluated the allelopathic potential of methanol extracts of F. japonica and F. × bohemica rhizomes and compared these with the effects of the individual knotweed phenols resveratrol, epicatechin and emodin, and their mixture. Rhizomes of both knotweeds contained similar amounts of epicatechin and emodin, with 24% higher resveratrol in F. × bohemica. Only the F. × bohemica methanol extract inhibited radish (Raphanus sativus) seed germination. After 3 days of treatments with 10% (w/v) extracts of both knotweeds, radish seedlings showed up to 70% shorter roots. In contrast, root growth of seedlings treated with the individual phenols resveratrol, epicatechin and emodin, and their mixture, was inhibited by up to 30%, similar to the 1% knotweed extracts. Biochemical parameters of oxidative stress also increased in the roots of treated seedlings, with high levels of malondialdehyde in particular indicating lipid peroxidation. Total antioxidative capacity was also increased in seedlings exposed to 0.6 mg/mL resveratrol and emodin. This study shows higher allelopathic potential of the knotweed methanol extracts compared to the individual phenols and their mixture.