Green Synthesized Silver Nanoparticles Using Anise (Pimpinella anisum L.) have Antibacterial Effects

Vaccine Research Pub Date : 2020-06-01 DOI:10.29252/vacres.7.1.17

Nassir Rashnaei, S. Siadat, A. Sepahi, M. Mirzaee, G. Bahramali, A. Joshaghani

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引用次数: 2

Abstract

Introduction: Silver nanoparticles are particles of silver with a size of 1 to 100 nm. These agents have various applications and particularly have received much attention for their antibacterial activity and their use in vaccine production. Among the various methods of synthesizing nanoparticles, using plants due to their high reducing capabilities and also their eco-friendliness is of interest. Methods: Silver nanoparticles (AgNPs) were synthesized using plant anise (Pimpinella anisum L.) and validated using UVspectrophotometer, transmission electron microscopy and Fourier transform infrared spectroscopy. The produced AgNPs were used against Escherichia coli, Salmonella typhimurium, Staphylococcus aureus and Enterococcus faecalis to examine their antibacterial activities via agar well diffusion, disk diffusion and minimum inhibitory concentration methods. Furthermore, AgNPs were used in combination with three antibiotic disks, namely, Ceftriaxone, Tetracycline and Gentamicin to seek any cooperative effect. Results: Antibacterial effects due to the synthesized AgNPs were observed toward E. coli, S. aureus, S. typhimurium in this order; however, E. faecalis showed the highest resistance to the synthesized AgNPs. Conclusion: AgNPs synthesized using anise had similar antibacterial effects as conventional antibiotics; however with potentially less side effects. Citation: Rashnaei N, Siadat S D, Akhavan Sepahi A, Mirzaee M, Bahramali G, Arab Joshaghani A. Green Synthesized Silver Nanoparticles Using Anise (Pimpinella anisum L.) have Antibacterial Effects. vacres. 2020; 7 (1) :17-24. DOI: 10.29252/vacres.7.1.17

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利用茴香（Pimpinella anisum L.）合成绿色银纳米粒子具有抗菌作用

简介：银纳米颗粒是银的颗粒，尺寸为1至100纳米。这些制剂具有多种应用，特别是由于其抗菌活性和在疫苗生产中的应用而受到广泛关注。在合成纳米颗粒的各种方法中，使用植物由于其高还原能力和生态友好性而备受关注。方法：用植物茴香（Pimpinella anisum L.）合成银纳米粒子，并用紫外分光光度计、透射电子显微镜和傅里叶变换红外光谱进行验证。将制备的AgNPs用于对抗大肠杆菌、鼠伤寒沙门氏菌、金黄色葡萄球菌和粪肠球菌，通过琼脂孔扩散法、圆盘扩散法和最小抑菌浓度法检测其抗菌活性。此外，AgNPs与三种抗生素片（即头孢曲松、四环素和庆大霉素）联合使用，以寻求任何协同作用。结果：合成的AgNPs对大肠杆菌、金黄色葡萄球菌、鼠伤寒杆菌的抗菌效果依次为：；然而，粪大肠杆菌对合成的AgNPs表现出最高的抗性。结论：茴香合成的AgNPs具有与传统抗生素相似的抗菌作用；然而潜在的副作用较小。引文：Rashnaei N，Siadat SD，Akhavan Sepahi A，Mirzaee M，Bahramali G，Arab Joshaghani A.使用茴香（Pimpinella anisum L.）合成的绿色银纳米粒子具有抗菌作用。真空。2020年；7（1）：17-24。DOI:10.29252/vacres.7.1.17

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Vaccine Research

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审稿时长

15 weeks