Sheep pestivirus in Morocco: sero-epidemiological and molecular study

IF 1.3 Q2 VETERINARY SCIENCES

Veterinary Record Open Pub Date : 2019-09-01 DOI:10.1136/vetreco-2018-000324

O. Fassi Fihri, Noâma Jammar, N. Amrani, I. El Berbri, Said Alali

{"title":"Sheep pestivirus in Morocco: sero-epidemiological and molecular study","authors":"O. Fassi Fihri, Noâma Jammar, N. Amrani, I. El Berbri, Said Alali","doi":"10.1136/vetreco-2018-000324","DOIUrl":null,"url":null,"abstract":"The present study is the first to investigate Border disease caused by the sheep pestivirus (SPV) in sheep herds in Morocco. Sero-epidemiological investigations were carried out in six regions of the Kingdom, known as important in terms of sheep breeding. A total of 760 blood samples were collected including aborted ewes from 28 randomly selected farms. The samples were analysed, for the determination of anti-pestivirus antibodies, using indirect ELISA technique. Next, reverse transcriptase PCR (RT-PCR) was conducted on serologically negative samples to identify possible persistently infected (PI) animals, through detection of specific RNA fragment. The results revealed an overall SPV seroprevalence in studied areas of 28.9%. The difference in seroprevalence between the six investigated regions was not statistically significant (p>0.05) and varied slightly from 20.9% to 37.5%. Furthermore, 93% of investigated farms were affected with an average seroprevalence of 22.7% (with a variation of 1%–74%). RT-PCR results were all negative, indicating the absence of PI animals in the tested samples. Nevertheless, the present study revealed that SPV is endemic in Morocco.","PeriodicalId":23565,"journal":{"name":"Veterinary Record Open","volume":" ","pages":""},"PeriodicalIF":1.3000,"publicationDate":"2019-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1136/vetreco-2018-000324","citationCount":"4","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Veterinary Record Open","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1136/vetreco-2018-000324","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"VETERINARY SCIENCES","Score":null,"Total":0}

引用次数: 4

Abstract

The present study is the first to investigate Border disease caused by the sheep pestivirus (SPV) in sheep herds in Morocco. Sero-epidemiological investigations were carried out in six regions of the Kingdom, known as important in terms of sheep breeding. A total of 760 blood samples were collected including aborted ewes from 28 randomly selected farms. The samples were analysed, for the determination of anti-pestivirus antibodies, using indirect ELISA technique. Next, reverse transcriptase PCR (RT-PCR) was conducted on serologically negative samples to identify possible persistently infected (PI) animals, through detection of specific RNA fragment. The results revealed an overall SPV seroprevalence in studied areas of 28.9%. The difference in seroprevalence between the six investigated regions was not statistically significant (p>0.05) and varied slightly from 20.9% to 37.5%. Furthermore, 93% of investigated farms were affected with an average seroprevalence of 22.7% (with a variation of 1%–74%). RT-PCR results were all negative, indicating the absence of PI animals in the tested samples. Nevertheless, the present study revealed that SPV is endemic in Morocco.

查看原文本刊更多论文

摩洛哥绵羊鼠疫病毒的血清流行病学和分子研究

本研究首次调查了绵羊鼠疫病毒（SPV）在摩洛哥绵羊群中引起的边境病。在王国的六个地区进行了血清流行病学调查，这些地区在绵羊饲养方面很重要。共从28个随机选择的农场采集了760份血液样本，其中包括流产的母羊。使用间接ELISA技术对样品进行分析，以测定抗鼠疫病毒抗体。接下来，对血清学阴性样本进行逆转录聚合酶链式反应（RT-PCR），通过检测特异性RNA片段来识别可能的持续感染（PI）动物。结果显示，研究地区的SPV总血清流行率为28.9%。六个调查地区之间的血清流行率差异无统计学意义（p>0.05），从20.9%到37.5%略有变化。此外，93%的调查农场受到影响，平均血清流行率22.7%（变化1%-74%）。RT-PCR结果均为阴性，表明试验样品中没有PI动物。然而，目前的研究表明，SPV在摩洛哥是地方病。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Veterinary Record Open VETERINARY SCIENCES-

CiteScore

3.00

自引率

0.00%

发文量

审稿时长

19 weeks

期刊介绍： Veterinary Record Open is a journal dedicated to publishing specialist veterinary research across a range of topic areas including those of a more niche and specialist nature to that considered in the weekly Vet Record. Research from all disciplines of veterinary interest will be considered. It is an Open Access journal of the British Veterinary Association.