Isolation, characterization, and molecular identification of probiotics showing promising hypoglycemia operating activities

IF 0.7 Q4 PHARMACOLOGY & PHARMACY
Abdel-Whhab Hegazy, A. El-Waseif, D. Maany
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引用次数: 0

Abstract

Background Probiotics are the most useful microorganisms for animal and human health; they are used in the pharmaceutical and food industries for many products that enhance digestion and immunity. Objective The objective of our study was to isolate, characterize, and identify a probiotic bacterial strain and determine its hypoglycemia operating parameters. Materials and methods Our research was carried out through the isolation of probiotic colonies from milk samples on MRS medium. Bacterial isolates were characterized both morphologically and biochemically. The collected bacterial isolates were tested for their low pH tolerance on phosphate buffer pH 2.0 and bile salt tolerance in MRS-THIO liquid medium. Glucose assimilation activity was tested by measuring the residual glucose concentration on MRS liquid medium at 37°C after 24 and 48 h by GOD-PAP enzymatic colorimetric method. The initial glucose concentration was 500 mg/dl. The most potent isolate was identified by methods of 16S-rDNA sequencing. Results and conclusion Twenty-one bacterial isolates were isolated and characterized. Bacterial isolates showed the highest resistance to acidic pH 2.0 and they were bile-tolerant. Results of glucose assimilation showed that there was a marked increase in sugar consumption rate after 48 h more than 24 h in most of the bacterial isolates. The top 10 isolates were selected for the testing of the rest of the parameters. Results show that no noticeable differences were observed in the consumption of glucose in the low-glucose concentration, but with more glucose concentration more glucose consumption rate differences were recorded among organisms. The top two organisms that have the ability to reproduce and consume glucose even in high-glucose concentrations were Ab 9 and Ab 2 with results of glucose residual concentration of 108 and 124 mg/dl, respectively. The phylogenetic tree showed that the most potent isolate Ab 2 was identified as Lactobacillus brevis.
具有良好降糖作用的益生菌的分离、表征和分子鉴定
背景益生菌是对动物和人类健康最有用的微生物;它们被用于制药和食品行业,用于许多增强消化和免疫力的产品。目的本研究的目的是分离、表征和鉴定一种益生菌菌株,并确定其低血糖操作参数。材料和方法我们的研究是通过在MRS培养基上从牛奶样品中分离益生菌菌落来进行的。细菌分离物在形态和生化上都有特征。测试收集的细菌分离株在pH 2.0的磷酸盐缓冲液中的低pH耐受性和在MRS-THIO液体培养基中的胆盐耐受性。通过测量24和48小时后37°C下MRS液体培养基上的残余葡萄糖浓度来测试葡萄糖同化活性 h。初始葡萄糖浓度为500 mg/dl。通过16S rDNA测序方法鉴定出最有效的分离株。结果与结论共分离鉴定出21株细菌。细菌分离株对酸性pH 2.0表现出最高的抗性,并且具有胆汁耐受性。葡萄糖同化的结果表明,48小时后糖的消耗率显著增加 h超过24 h。选择前10个分离株进行其余参数的测试。结果表明,在低葡萄糖浓度下,葡萄糖消耗没有观察到显著差异,但随着葡萄糖浓度的增加,生物体之间的葡萄糖消耗率差异越来越大。即使在高葡萄糖浓度下也有能力繁殖和消耗葡萄糖的前两种生物是Ab 9和Ab 2,葡萄糖残留浓度分别为108和124 mg/dl。系统发育树表明,最有效的分离株Ab2被鉴定为短乳杆菌。
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来源期刊
Egyptian Pharmaceutical Journal
Egyptian Pharmaceutical Journal PHARMACOLOGY & PHARMACY-
CiteScore
1.10
自引率
0.00%
发文量
37
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