Efficient solvent suppression with adiabatic inversion for 1H-detected solid-state NMR

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2021-10-21 DOI:10.1007/s10858-021-00384-8

Tatsuya Matsunaga, Ryotaro Okabe, Yoshitaka Ishii

引用次数: 4

Abstract

This study introduces a conceptually new solvent suppression scheme with adiabatic inversion pulses for ¹H-detected multidimensional solid-state NMR (SSNMR) of biomolecules and other systems, which is termed “Solvent suppression of Liquid signal with Adiabatic Pulse” (SLAP). ¹H-detected 2D ¹³C/¹H SSNMR data of uniformly ¹³C- and ¹⁵N-labeled GB1 sample using ultra-fast magic angle spinning at a spinning rate of 60 kHz demonstrated that the SLAP scheme showed up to 3.5-fold better solvent suppression performance over a traditional solvent-suppression scheme for SSNMR, MISSISSIPPI (Zhou and Rienstra, J Magn Reson 192:167–172, 2008) with 2/3 of the average RF power.

Abstract Image

查看原文本刊更多论文

高效溶剂抑制与绝热反演的1h检测固态核磁共振

本研究提出了一种概念上的新型溶剂抑制方案，即“溶剂抑制液体信号与绝热脉冲”(SLAP)，用于生物分子和其他体系的1h检测多维固态核磁共振(SSNMR)。采用超快魔角纺丝，在60 kHz的纺丝速率下，对均匀13C-和15n -标记的GB1样品进行了二维13C/1H SSNMR数据检测，结果表明，在平均射频功率为2/3的情况下，SLAP方案比传统的SSNMR溶剂抑制方案的溶剂抑制性能提高了3.5倍(Zhou和Rienstra, J Magn Reson 192:167-172, 2008)。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.