Effect of Human Bone Marrow Mesenchymal Stem Cells on Burn Healing in Granulation Tissue Formation in Diabetic Rats

Jurnal Kesehatan Andalas Pub Date : 2023-04-18 DOI:10.25077/jka.v11i3.2089

Gusti Revilla, Deddy Saputra, Dini Nurhasanah

{"title":"Effect of Human Bone Marrow Mesenchymal Stem Cells on Burn Healing in Granulation Tissue Formation in Diabetic Rats","authors":"Gusti Revilla, Deddy Saputra, Dini Nurhasanah","doi":"10.25077/jka.v11i3.2089","DOIUrl":null,"url":null,"abstract":"Burns with diabetes mellitus can interfere with the wound-healing process. Mesenchymal stem cells have been studied to accelerate the healing of burns, one of which originates from the bone marrow. Objective: To determined the effect of human bone marrow mesenchymal stem cells (hBM-MSCs) on burn wound healing in granulation tissue formation in Diabetes Mellitus rats. Methods: This study was an experimental study with a post-test-only control group design, using 30 rats divided into two groups, the control group Phosphate Buffer Saline (PBS) and the treatment group (hBM-MSCs). Mice were induced by alloxan to cause hyperglycemia. Burns are made using a heated plate. Tissue collection was carried out after the termination of the experimental animals on the 3rd, 7th and 14th days and then histological preparations were made to assess the formation of granulation tissue. Data were analyzed using the two-way ANOVA test. Results: There was an increase in the number of macrophages, fibroblasts and new blood vessels after being given hBM-MSCs. On day 14 neutrophils and lymphocytes on day 3, there was no increase. Statistical analysis showed a significant increase in lymphocytes, macrophages, fibroblasts and new blood vessels. Conclusion: Human bone marrow mesenchymal stem cells can increase the formation of granulation tissue by increasing the number of macrophages, fibroblasts and new blood vessels in diabetic rats.Keywords: burn wound, diabetic rat, human bone marrow mesenchymal stem cells, granulation tissue","PeriodicalId":30736,"journal":{"name":"Jurnal Kesehatan Andalas","volume":" ","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2023-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Jurnal Kesehatan Andalas","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.25077/jka.v11i3.2089","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}

引用次数: 0

Abstract

Burns with diabetes mellitus can interfere with the wound-healing process. Mesenchymal stem cells have been studied to accelerate the healing of burns, one of which originates from the bone marrow. Objective: To determined the effect of human bone marrow mesenchymal stem cells (hBM-MSCs) on burn wound healing in granulation tissue formation in Diabetes Mellitus rats. Methods: This study was an experimental study with a post-test-only control group design, using 30 rats divided into two groups, the control group Phosphate Buffer Saline (PBS) and the treatment group (hBM-MSCs). Mice were induced by alloxan to cause hyperglycemia. Burns are made using a heated plate. Tissue collection was carried out after the termination of the experimental animals on the 3rd, 7th and 14th days and then histological preparations were made to assess the formation of granulation tissue. Data were analyzed using the two-way ANOVA test. Results: There was an increase in the number of macrophages, fibroblasts and new blood vessels after being given hBM-MSCs. On day 14 neutrophils and lymphocytes on day 3, there was no increase. Statistical analysis showed a significant increase in lymphocytes, macrophages, fibroblasts and new blood vessels. Conclusion: Human bone marrow mesenchymal stem cells can increase the formation of granulation tissue by increasing the number of macrophages, fibroblasts and new blood vessels in diabetic rats.Keywords: burn wound, diabetic rat, human bone marrow mesenchymal stem cells, granulation tissue

查看原文本刊更多论文

人骨髓间充质干细胞对糖尿病大鼠烧伤愈合及颗粒组织形成的影响

糖尿病烧伤会干扰伤口愈合过程。间充质干细胞已被研究用于加速烧伤的愈合，其中一种来自骨髓。目的：观察人骨髓间充质干细胞（hBM-MSCs）对糖尿病大鼠烧伤创面肉芽组织形成的影响。方法：本研究采用试验后对照组设计，将30只大鼠分为两组，对照组磷酸盐缓冲液（PBS）和治疗组hBM-MSCs。四氧嘧啶诱导小鼠产生高血糖。烧伤是用加热的盘子做的。在实验动物终止后第3、7和14天进行组织收集，然后进行组织学准备以评估肉芽组织的形成。使用双向ANOVA检验对数据进行分析。结果：给予hBM-MSCs后，巨噬细胞、成纤维细胞和新生血管的数量增加。在第14天中性粒细胞和第3天淋巴细胞没有增加。统计分析显示淋巴细胞、巨噬细胞、成纤维细胞和新生血管显著增加。结论：人骨髓间充质干细胞可通过增加糖尿病大鼠巨噬细胞、成纤维细胞和新生血管的数量来促进肉芽组织的形成。关键词：烧伤创面；糖尿病大鼠；人骨髓间充质干细胞；肉芽组织

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Jurnal Kesehatan Andalas

自引率

0.00%

发文量

审稿时长

24 weeks