IL-6 Promotes Hepatocellular Carcinoma Invasion by Releasing Exosomal miR-133a-3p

IF 2 4区医学 Q3 GASTROENTEROLOGY & HEPATOLOGY

Gastroenterology Research and Practice Pub Date : 2022-04-06 DOI:10.1155/2022/4589163

Xudong Ren, Yu Zhou, Yunling Luo, Chaoqun Wang, Anna Pan, Y. Ju, Haoting Sun, Zhifei Lin, Beiyuan Hu, G. Sun, Wenwei Zhu, L. Hong

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引用次数: 1

Abstract

Interleukin-6 (IL-6), an important inflammatory cytokine, is a key factor regulating cancer metastasis. Cancer cells can modulate their tumorigenic abilities by sorting specific microRNAs (miRNAs) as exosomes into the tumor microenvironment. The relationship between IL-6 and exosomal miRNAs related to hepatocellular carcinoma (HCC) metastasis remains to be elucidated. We examined the metastatic ability of HCC cells after IL-6 treatment and found that miR-133a-3p was sorted into exosomes after IL-6 stimulation and was subsequently released into the tumor microenvironment. In vitro analysis confirmed that exosomal miR-133a-3p acted as a tumor suppressor in HCC. Bioinformatic analysis revealed several signaling pathways and hub genes (CREB1, VCP, CALM1, and YES1) regulated by miR-133a-3p. Survival curves further verified the important roles of hub genes in the prognosis of patients with HCC. It is envisaged that the IL-6/miR-133a-3p axis may be related to the activation of CREB1, VCP, CALM1, and YES1. Our findings provide new insights into the role of exosomal miRNA-mediated tumor progression under inflammatory conditions.

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IL-6通过释放外泌体miR-133a-3p促进肝癌侵袭

白细胞介素-6 (IL-6)是一种重要的炎症细胞因子，是调节肿瘤转移的关键因子。癌细胞可以通过将特定的microrna (mirna)作为外泌体分选到肿瘤微环境中来调节其致瘤能力。IL-6与肝细胞癌(HCC)转移相关的外泌体mirna之间的关系仍有待阐明。我们检测了IL-6处理后HCC细胞的转移能力，发现miR-133a-3p在IL-6刺激后被分选到外泌体中，随后被释放到肿瘤微环境中。体外分析证实外泌体miR-133a-3p在HCC中起肿瘤抑制作用。生物信息学分析揭示了miR-133a-3p调控的几种信号通路和枢纽基因(CREB1、VCP、CALM1和YES1)。生存曲线进一步验证了枢纽基因在HCC患者预后中的重要作用。据推测，IL-6/miR-133a-3p轴可能与CREB1、VCP、CALM1和YES1的激活有关。我们的发现为炎症条件下外泌体mirna介导的肿瘤进展的作用提供了新的见解。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Gastroenterology Research and Practice GASTROENTEROLOGY & HEPATOLOGY-

CiteScore

4.40

自引率

0.00%

发文量

审稿时长

1 months

期刊介绍： Gastroenterology Research and Practice is a peer-reviewed, Open Access journal which publishes original research articles, review articles and clinical studies based on all areas of gastroenterology, hepatology, pancreas and biliary, and related cancers. The journal welcomes submissions on the physiology, pathophysiology, etiology, diagnosis and therapy of gastrointestinal diseases. The aim of the journal is to provide cutting edge research related to the field of gastroenterology, as well as digestive diseases and disorders. Topics of interest include: Management of pancreatic diseases Third space endoscopy Endoscopic resection Therapeutic endoscopy Therapeutic endosonography.