Effects of Prestretch on Neonatal Peripheral Nerve: An In Vitro Study

IF 1.1 Q4 CLINICAL NEUROLOGY
Anita Singh, T. Majmudar, R. Magee, B. Gonik, Sriram Balasubramanian
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Abstract

Background  Characterizing the biomechanical failure responses of neonatal peripheral nerves is critical in understanding stretch-related peripheral nerve injury mechanisms in neonates. Objective  This in vitro study investigated the effects of prestretch magnitude and duration on the biomechanical failure behavior of neonatal piglet brachial plexus (BP) and tibial nerves. Methods  BP and tibial nerves from 32 neonatal piglets were harvested and prestretched to 0, 10, or 20% strain for 90 or 300 seconds. These prestretched samples were then subjected to tensile loading until failure. Failure stress and strain were calculated from the obtained load-displacement data. Results  Prestretch magnitude significantly affected failure stress but not the failure strain. BP nerves prestretched to 10 or 20% strain, exhibiting significantly lower failure stress than those prestretched to 0% strain for both prestretch durations (90 and 300 seconds). Likewise, tibial nerves prestretched to 10 or 20% strain for 300 seconds, exhibiting significantly lower failure stress than the 0% prestretch group. An effect of prestretch duration on failure stress was also observed in the BP nerves when subjected to 20% prestretch strain such that the failure stress was significantly lower for 300 seconds group than 90 seconds group. No significant differences in the failure strains were observed. When comparing BP and tibial nerve failure responses, significantly higher failure stress was reported in tibial nerve prestretched to 20% strain for 300 seconds than BP nerve. Conclusion  These data suggest that neonatal peripheral nerves exhibit lower injury thresholds with increasing prestretch magnitude and duration while exhibiting regional differences.
预拉伸对新生儿周围神经影响的体外研究
背景 表征新生儿周围神经的生物力学失效反应对于理解新生儿拉伸相关的周围神经损伤机制至关重要。客观的 本体外研究探讨了预拉伸幅度和持续时间对新生仔猪臂丛神经和胫神经生物力学失效行为的影响。方法 采集32只新生仔猪的BP和胫骨神经,并将其预拉伸至0%、10%或20%的菌株90或300 秒。然后对这些预拉伸的样品进行拉伸加载直到失效。根据所获得的载荷-位移数据计算失效应力和应变。后果 预应力大小对破坏应力有显著影响,但对破坏应变没有影响。BP神经预拉伸至10%或20%应变,在两个预拉伸持续时间(90和300 秒)。同样,胫骨神经预拉伸至10%或20%的应变300 秒,表现出比0%预拉伸组显著更低的失效应力。当受到20%的预拉伸应变时,在BP神经中也观察到预拉伸持续时间对失效应力的影响,使得300 秒组大于90 秒组。未观察到失效菌株的显著差异。当比较BP和胫神经衰竭反应时,据报道,在预拉伸至20%应变的300 秒比BP神经。结论 这些数据表明,随着预拉伸幅度和持续时间的增加,新生儿外周神经表现出较低的损伤阈值,同时表现出区域差异。
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来源期刊
CiteScore
1.70
自引率
14.30%
发文量
6
审稿时长
12 weeks
期刊介绍: JBPPNI is an open access, peer-reviewed online journal that will encompass all aspects of basic and clinical research findings, in the area of brachial plexus and peripheral nerve injury. Injury in this context refers to congenital, inflammatory, traumatic, degenerative and neoplastic processes, including neurofibromatosis. Papers on diagnostic and imaging aspects of the peripheral nervous system are welcomed as well. The peripheral nervous system is unique in its complexity and scope of influence. There are areas of interest in the anatomy, physiology, metabolism, phylogeny, and limb growth tropism of peripheral nerves.
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