Colpomenia species from south and south-eastern Australia (Ectocarpales, Phaeophyceae): a DNA barcoding approach

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2021-10-14 DOI:10.1071/SB21021

N. Martins, C. Gurgel, Tracey M. Spokes, V. Cassano

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引用次数: 1

Abstract

Abstract. Defining species in the brown algal genus Colpomenia is a challenging endeavour because of their morphological similarity, overlapping phenotypic variation, the absence of conspicuous diagnostic characters, and often lack of reproductive structures crucial for their identification. Thus, the use of molecular tools has become widely used to study Colpomenia taxonomy and evolution. The following four Colpomenia species are described along the Australian coast: C. claytoniae M.Boo, K.M.Lee, G.Y.Cho & W.Nelson, C. ecuticulata M.J.Parsons, C. peregrina Sauvageau, and C. sinuosa (Mertens ex Roth) Derbès & Solier. The objective of this study was to assess the diversity of Colpomenia species in southern and south-eastern Australia by using DNA barcoding techniques and single-marker species delimitation methods. We generated 44 new COI-5P DNA sequences from nine different populations across three Australian states (South Australia, Victoria and Tasmania), and applied 13 variations of four species delimitation methods (ABDG, SPN, PTP, GMYC). Our results recognised three Colpomenia species in the region, namely, C. sinuosa, C. claytoniae, and C. peregrina. Colpomenia sinuosa is the most widely distributed species in Australia. Colpomenia peregrina and C. claytoniae presented high levels of intraspecific genetic divergence. We did not find C. ecuticulata, although it has been previously reported from nearby our sampling area.

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澳大利亚南部和东南部的水蛭属植物(石竹科，石竹科):DNA条形码方法

摘要定义棕藻属的物种是一项具有挑战性的工作，因为它们的形态相似，重叠的表型变异，缺乏显著的诊断特征，并且通常缺乏对其识别至关重要的生殖结构。因此，分子工具的使用已被广泛用于研究水蛭属植物的分类和进化。以下四种粪门属在澳大利亚海岸被发现:C. claytoniae M.Boo, K.M.Lee, G.Y.Cho & w.l nelson, C. ecuticulata m.j.s parsons, C. peregrina Sauvageau和C. sinuosa (Mertens ex Roth) derb & Solier。采用DNA条形码技术和单标记物种划分方法，对澳大利亚南部和东南部的水蛭属植物物种多样性进行了研究。我们从澳大利亚3个州(南澳大利亚州、维多利亚州和塔斯马尼亚州)的9个不同种群中获得了44条新的COI-5P DNA序列，并应用了4种物种划分方法(ABDG、SPN、PTP、GMYC)的13种变体。结果表明，该地区存在3种粪菌属，分别为C. sinuosa、C. claytoniae和C. peregrina。sinuosa是澳大利亚分布最广的一种。游隼鸟和克莱顿鸟表现出高度的种内遗传分化。虽然以前在我们的采样区附近有过报道，但我们没有发现锥虫。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.