Production of a diploid hybrid with fast growth performance derived from the distant hybridization of Hypophthalmichthys nobilis (female) × Megalobrama amblycephala (male)

Reproduction and breeding Pub Date : 2022-06-01 DOI:10.1016/j.repbre.2022.05.002

Yude Wang , Yaxin Luo , Chen Geng, An'min Liao, Rurong Zhao, Huifang Tan, Jiajun Yao, Shi Wang, Kaikun Luo, Qinbo Qin, Chun Zhang, Min Tao, Shaojun Liu

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引用次数: 2

Abstract

Based on the distant hybridization derived from Hypophthalmichthys nobilis (bighead carp, BIC, 2n = 48) (♀) × Megalobrama amblycephala (blunt snout bream, BSB, 2n = 48) (♂), we obtained a novel hybrid (BB) in this study. We comparatively investigated the morphological traits, chromosome numbers, DNA contents, microsatellite patterns, and DNA variations in 5S rDNA and Hox genes. The chromosome and flow cytometer results indicated that BB was a diploid hybrid with 48 chromosomes. We confirmed successful hybridization with microsatellite DNA markers and analyzed the Hox gene family in the parental and hybrid lines. The sequences of 5S rDNA in BB mapped to those in BIC and BSB, indicating that the genetic materials of BB were derived from BIC and BSB. The breeding experiment showed that BB had the characteristics of herbivore and fast growth. This diploid hybrid is of great significance for fish genetic breeding and applied research.

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矮眼鱼(雌性)与巨头鱼(雄性)远缘杂交获得快速生长的二倍体杂种

本研究以Hypophthalmichthys nobilis (high - head carp, BIC, 2n = 48)(♀)与Megalobrama amblycephala (blunt snout bream, BSB, 2n = 48)(♂)为亲本，获得了一种新的杂交品种(BB)。我们比较研究了5S rDNA和Hox基因的形态特征、染色体数目、DNA含量、微卫星模式和DNA变异。染色体和流式细胞仪检测结果表明BB为二倍体杂交种，有48条染色体。我们用微卫星DNA标记证实了成功的杂交，并分析了亲本和杂交种的Hox基因家族。BB的5S rDNA序列与BIC和BSB的5S rDNA序列一致，表明BB的遗传物质来源于BIC和BSB。育种试验表明，BB具有草食性、生长快的特点。该二倍体杂交种对鱼类遗传育种和应用研究具有重要意义。

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来源期刊

Reproduction and breeding

CiteScore

2.40

自引率

0.00%

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