Molecular epidemiology of piliated pneumococcal isolates at a major tertiary hospital in the Klang Valley, Malaysia

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2023-02-01 DOI:10.4103/1995-7645.370150

N. Dzaraly, M. Desa, AbdulRahman Muthanna, S. Masri, N. Taib, N. Baharin, N. Sulaiman, Nurul Rahman, N. Palanisamy, Z. Suhaili, Semiat Yusuff, N. Rahman, T. Soh, Fatimah Abdullah8

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引用次数: 0

Abstract

Objective: To characterise a collection of pili-carrying and none pili-carrying pneumococcal isolates of clinical origin for serotypes, antibiotic resistance and genotype. Methods: In total, 42 clinical isolates were collected between October 2017 and December 2019. Those isolates were analysed for antimicrobial susceptibility, serotype distribution, detection of pneumococcal virulence and pilus genes. Multilocus sequence typing was performed only for piliated isolates, followed by phylogenetic analysis. Results: The common isolation sites among the pneumococcal isolates were tracheal aspirate (28.6%), blood (26.2%), and sputum (23.8%). Fifty percent isolates were resistant to erythromycin, tetracycline (50.0%) and trimethoprim-sulfamethoxazole (43.0%). The most frequent were serotypes 19F (28.6%), 6A/B (23.8%) and 19A (14.3%). Piliated isolates were detected in a small proportion (33.3%); 64.3% were multidrug-resistant. ST320 was the prevalent sequence type among the piliated isolates and genetically related to the Pneumococcal Molecular Epidemiology Network clones Taiwan19F-14 (CC271). In the phylogenetic analysis, some piliated isolates showed a close association having similar ST320, carrying serotype 19A and both pilus genes indicating their clonal spread. Conclusions: Pneumococcal lineages of piliated isolates have been globally disseminated and pili could have played a role in the spread of antibiotic resistant clones.

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马来西亚巴生谷一家大型三级医院中肺炎球菌毛状分离株的分子流行病学研究

目的：对临床来源的携带菌毛和不携带菌毛的肺炎球菌分离株的血清型、抗生素耐药性和基因型进行鉴定。方法：2017年10月至2019年12月，共收集42株临床分离株。对这些分离株进行了抗菌药物敏感性、血清型分布、肺炎球菌毒力检测和菌毛基因分析。只对菌毛分离株进行多基因座序列分型，然后进行系统发育分析。结果：肺炎球菌分离株中常见的分离位点为气管分泌物（28.6%）、血液（26.2%）和痰（23.8%），50%的分离株对红霉素、四环素（50.0%）和甲氧苄啶-磺胺甲恶唑（43.0%）具有耐药性，最常见的是血清型19F（28.6%、23.8%和14.3%）；耐多药占64.3%。ST320是菌毛分离株中的流行序列型，与肺炎球菌分子流行病学网络克隆Taiwan19F-14（CC271）具有遗传相关性。在系统发育分析中，一些菌毛分离株显示出密切的相关性，具有相似的ST320，携带血清型19A和两个菌毛基因，表明它们的克隆传播。结论：菌毛分离株的肺炎球菌谱系已经在全球传播，菌毛可能在抗生素耐药性克隆的传播中发挥了作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.