Light regimen-induced variability of photosynthetic pigments and UV-B absorbing compounds in Luzula sylvatica from Arcto-Alpine tundra

IF 0.5 Q4 ECOLOGY

Czech Polar Reports Pub Date : 2020-07-01 DOI:10.5817/CPR2020-2-20

M. Barták, A. Orekhova, J. Nezval, M. Oravec, J. Hájek, V. Špunda, J. Tříska, M. Bednaříková, G. Giudici, Radek Pech

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引用次数: 1

Abstract

The aim of this study was to evaluate the effects of different in situ light regimen on ecophysiological parameters of Luzula sylvatica leaves. Plants of L. sylvatica grown under natural sunny and shade conditions in arcto-alpine tundra were analyzed with respect to their leaf anatomy, content of photosynthetic pigments, UV absorbing compounds and phenanthrenoid compounds. Relationship between chlorophyll concentrations (Chl a+b ) and SPAD values was determined for sun and shade leaves measured repeatedly within summer and autumn seasons 2019 and 2020. Pooled data showed curvilinear Chl a+b to SPAD relationship with the highest Chl a+b and SPAD values found for shade leaves. Sun leaves had higher UV-B absorbing compounds contents than shade ones. The HPLC-DAD analysis revealed significant amount of soluble flavonoids in Luzula sylvatica leaves, amongst others the flavone-luteolin and its derivatives ( e.g. tentatively identified luteolin-methyl-glucoside and luteolin-glucoside). The accumulation of luteolin based compounds in sun acclimated leaves is also plausible explanation for the higher antioxidant activity determined in sun leaf extraxts. Such response of flavonoid metabolism may help L.S. to cope with excessive-light stress through UV-attenuation mechanism and ROS scavanging. Additionally, phenanthrenoid compounds contents in L. sylvatica leaves were determined. Altogether, 9 phenanthrenoid compounds were identified by HPLC-HRMS. Their content was markedly different (up to the factor of 5) between sun and shade leaves of L.sylvatica .

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光照诱导的Arcto Alpine苔原银杉光合色素和UV-B吸收化合物的变异

本研究的目的是评价不同的原位光照方案对木犀叶片生态生理参数的影响。分析了在自然向阳和遮荫条件下生长在弧形高山苔原上的L.sylvatica植物的叶片解剖结构、光合色素、紫外线吸收化合物和类菲化合物的含量。测定了2019年和2020年夏秋季节反复测量的阳光和遮荫叶片的叶绿素浓度（Chl a+b）与SPAD值之间的关系。汇集的数据显示Chl a+b与SPAD的曲线关系，遮荫叶的Chl a+b和SPAD值最高。太阳叶的UV-B吸收化合物含量高于遮荫叶。HPLC-DAD分析显示，木犀草叶中含有大量可溶性黄酮类化合物，其中包括黄酮木犀草素及其衍生物（例如，初步鉴定的木犀草甲糖苷和木犀草苷糖苷）。木犀草素基化合物在阳光适应的叶片中的积累也是阳光叶片提取物中抗氧化活性较高的合理解释。类黄酮代谢的这种反应可能有助于L.S.通过紫外线衰减机制和ROS清除来应对过度的光胁迫。此外，还测定了樟树叶片中类菲化合物的含量。HPLC-HRMS共鉴定出9个类菲化合物。其含量在L.sylvatica的遮阳叶和向阳叶之间存在显著差异（高达5倍）。

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来源期刊

Czech Polar Reports Agricultural and Biological Sciences-Agricultural and Biological Sciences (all)

CiteScore

1.30

自引率

10.00%

发文量

期刊介绍： Czech Polar Reports is an international, multidisciplinary, peer-reviewed journal. It is issued 2 times a year. The journal is dedicated to provide original research papers for sciences related to the polar regions and other planets with polar analogues. Czech Polar Reports covers the disciplines listed below. polar paleontology, geology, geochemistry, geomorphology, glaciology, climatology, hydrology, pedology, biochemistry, ecology, environmental science, microbiology, plant and animal biology including marine biology.