Antioxidant activity and inhibitory effect of curcumin on some enzymes involved in several diseases: Acetylcholinesterase, butyrylcholinesterase, α-glucosidase and tyrosinase

Q4 Pharmacology, Toxicology and Pharmaceutics

Current Enzyme Inhibition Pub Date : 2022-06-02 DOI:10.2174/1573408018666220602091615

Seghira Bisset, W. Sobhi, C. Bensouici, A. Khenchouche

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引用次数: 3

Abstract

This study aims the investigation of the potential effect of pure curcumin on the inhibition of different enzymes which are involved in several diseases. Several chronic diseases such as Alzheimer’s, diabetes, and Parkinson’s disease are related to oxidative stress and enzyme activity. Today, various plant origin products are stated as beneficial against several chronic diseases with secondary metabolites such as phenolic compounds. Curcumin, a polyphenol yellow-orange pigment present in turmeric spices, has a wide range of biological activities, with quite a safety. This study was aimed to investigate the antioxidant and inhibitory potential against key enzymes involved in human pathology, namely Alzheimer's disease (Acetylcholinesterase (AChE), and Butyrylcholinesterase (BChE)), diabetes (α-glucosidase) and hyperpigmentation and Parkinson’s diseases (Tyrosinase) of curcumin. 1,1-diphenyl-2-picrylhydrazyl free radical (DPPH●) and hydrogen peroxide radicals (H2O2) assays were used to evaluate the antioxidant capacity of curcumin, enzyme inhibitory activity was evaluated using in vitro standard procedures. Curcumin exhibited an excellent antioxidant effect with an IC50 value significantly less than the reference, Vit C. In enzyme inhibitory activity, curcumin demonstrated excellent inhibitory activity against AChE, BChE and α-glucosidase. The finding showed that curcumin was significantly less than the reference galantamine against AChE, but was significantly more than the references galantamine and acarbose against BChE and α-glucosidase, respectively. Whereas for anti-tyrosinase activity, curcumin displayed weak inhibitory activity compared with the standard inhibitor, Kojic acid.

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姜黄素对乙酰胆碱酯酶、丁基胆碱酯酶、α-葡萄糖苷酶和酪氨酸酶的抗氧化活性及抑制作用

本研究旨在探讨纯姜黄素对几种疾病中涉及的不同酶的抑制作用。一些慢性疾病，如阿尔茨海默病、糖尿病和帕金森病都与氧化应激和酶活性有关。今天，各种植物源产品被认为对几种慢性疾病有益，其次生代谢物如酚类化合物。姜黄素是一种存在于姜黄香料中的多酚类黄橙色色素，具有广泛的生物活性，具有相当的安全性。本研究旨在探讨姜黄素对阿尔茨海默病(乙酰胆碱酯酶(AChE)和丁基胆碱酯酶(BChE))、糖尿病(α-葡萄糖苷酶)、色素沉着和帕金森病(酪氨酸酶)等人类病理关键酶的抗氧化和抑制作用。采用1,1-二苯基-2-吡啶肼基自由基(DPPH)和过氧化氢自由基(H2O2)测定法评价姜黄素的抗氧化能力，采用体外标准程序评价姜黄素的酶抑制活性。姜黄素表现出较好的抗氧化作用，其IC50值显著低于对照物Vit c。在酶抑制活性方面，姜黄素对AChE、BChE和α-葡萄糖苷酶均表现出较好的抑制活性。结果表明，姜黄素对乙酰胆碱酯酶的抑制作用显著低于对照物加兰他敏，但对BChE和α-葡萄糖苷酶的抑制作用显著高于对照物加兰他敏和阿卡波糖。姜黄素对酪氨酸酶的抑制作用弱于标准抑制剂曲酸。

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来源期刊

Current Enzyme Inhibition Pharmacology, Toxicology and Pharmaceutics-Drug Discovery

CiteScore

1.30

自引率

0.00%

发文量

期刊介绍： Current Enzyme Inhibition aims to publish all the latest and outstanding developments in enzyme inhibition studies with regards to the mechanisms of inhibitory processes of enzymes, recognition of active sites, and the discovery of agonists and antagonists, leading to the design and development of new drugs of significant therapeutic value. Each issue contains a series of timely, in-depth reviews written by leaders in the field, covering a range of enzymes that can be exploited for drug development. Current Enzyme Inhibition is an essential journal for every pharmaceutical and medicinal chemist who wishes to have up-to-date knowledge about each and every development in the study of enzyme inhibition.