Analysis of FOXE1 rs4460498 and GSTP-1 I105V associated with non syndromic cleft lip and palate among Deutero Malay Subrace in Indonesia

Padjadjaran Journal of Dentistry Pub Date : 2022-11-30 DOI:10.24198/pjd.vol34no3.39296

S. Nasroen, F. Meliawaty, Tichvy Tammama, Putri Indriani Prasdiaz, Tias Choirunnisa, A. Maskoen

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Abstract

ABSTRACTIntroduction: FOXE1 rs4460498 and GSTP-1 I105V gene polymorphisms are suspected of having a role in some of the non-syndromic cleft lip and palate (NS CLP) populations worldwide. This study aims to analyze FOXE1 rs4460498 and GSTP-1 I105V polymorphisms associated with NS CLP as the risk factor among Deutero Malay Subrace in Indonesia. Methods: This study was a case-control design, using samples from the venous blood of 102 NS CLP subjects and 102 healthy control subjects. After DNA was extracted, the PCR-RFLPs method was performed using TasI restriction enzyme on 100 blood samples of FOXE1 rs4460498 group and Alw26I restriction enzyme on 105 blood samples of the GSTP-1 I105V group. The Chi-Square test was used with the Kolmogorov Smirnov and Exact Fisher alternatives. Results: T mutant allele (OR= 0.926, p>0.05) and CT genotype (OR= 0.0, p>0.05) of FOXE1 rs4460498 and the G mutant allele (OR= 0.988,p>0.05) and AG genotype (OR= 0.675,p>0.05) of the GSTP-1 I105V are not the risks of NS CLP. Conclusion: FOXE1 rs4460498 and GSTP-1 I105V gene polymorphisms are not associated with non-syndromic cleft lip and palate among Deutero Malay Subrace in the Indonesian population.Key words: deutero malay; FOXE1 rs4460498; GSTP-1 I105V; NS CLP

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印尼马来族非综合征性唇腭裂相关基因FOXE1 rs4460498和GSTP-1 I105V分析

摘要简介:FOXE1 rs4460498和GSTP-1 I105V基因多态性被怀疑在世界上一些非综合征性唇腭裂(NS CLP)人群中起作用。本研究旨在分析FOXE1 rs4460498和GSTP-1 I105V多态性与NS CLP作为Deutero马来印尼Subrace之间的风险因素。方法:本研究采用病例-对照设计，取NS - CLP患者102例和健康对照102例静脉血。提取DNA后，用TasI酶对100份FOXE1 rs4460498组血样进行PCR-RFLPs分析，用Alw26I酶对105份GSTP-1 I105V组血样进行PCR-RFLPs分析。卡方检验与Kolmogorov - Smirnov和Exact Fisher替代方案一起使用。结果FOXE1 rs4460498的T突变等位基因(OR= 0.926, p>0.05)、CT基因型(OR= 0.0, p>0.05)和GSTP-1 I105V的G突变等位基因(OR= 0.988,p>0.05)、AG基因型(OR= 0.675,p>0.05)与NS CLP无关。结论:FOXE1 rs4460498和GSTP-1 I105V基因多态性与印尼人群非综合征性唇腭裂无关。关键词:马来人;FOXE1 rs4460498;GSTP-1 I105V;NS CLP

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Padjadjaran Journal of Dentistry

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3 weeks