Calcium Signaling Involves Na+/H+ Exchanger and IP3 Receptor Activation in T. cruzi Epimastigotes

Melina Usorach, A. Gimenez, Micaela Peppino Margutti, G. Racagni, E. Machado
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Abstract

The calcium ion (Ca2+) plays a fundamental role in the metabolism and cell physiology of eukaryotic cells. In general, increases in cytosolic Ca2+ may come from both of the extracellular environment through specific channels and/or calcium release from intracellular stores. The mechanism by which the ion calcium (Ca2+) is released from intracellular stores in higher eukaryotes is well known; however, in lower eukaryotes is still a subject of study. In the present work, it was elucidated that Trypanosoma cruzi epimastigotes can release Ca2+ from intracellular stores in response to high osmolarity, in a process involving a protein kinase-regulated Na+/H+ exchanger present in the acidocalsisomes of the parasite. In addition, we demonstrated that epimastigote membranes are able to release Ca2+ in response to exogenous activators of both inositol 1,4,5-triphosphate (IP3) and Ryanodine receptors. Furthermore, we also summarize the involvement of calcium-related signaling pathways in biochemical and morphological changes triggered by hyperosmotic stress in T. cruzi epimastigotes.
克鲁兹乳杆菌钙信号传导与Na+/H+交换和IP3受体激活
钙离子(Ca2+)在真核细胞的代谢和细胞生理中起着重要作用。一般来说,细胞质Ca2+的增加可能来自细胞外环境通过特定通道和/或钙从细胞内储存释放。在高等真核生物中,钙离子(Ca2+)从细胞内储存中释放的机制是众所周知的;然而,在低等真核生物中仍是一个研究课题。在目前的工作中,研究人员阐明了克氏锥虫表皮毛囊体可以在高渗透压下从细胞内释放Ca2+,这一过程涉及到一种蛋白激酶调节的Na+/H+交换,这种交换存在于寄生虫的酸溶体中。此外,我们证明了外源性肌醇1,4,5-三磷酸(IP3)和Ryanodine受体的激活剂能够释放Ca2+。此外,我们还总结了钙相关信号通路在高渗胁迫引发的克氏绦虫生化和形态变化中的作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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