Assessing garden pea germplasm for powdery mildew resistance through disease phenotyping and genotyping using molecular markers

IF 2.2 Q3 GENETICS & HEREDITY
Priyambada Sharada, Ragiba Makandar
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Abstract

Erysiphe pisi is one among the other causal agents of powdery mildew infection in garden pea. So far, the resistance genes comprising two recessive (er1 and er2) and one dominant (Er3) have been reported to confer resistance to powdery mildew in garden pea. A set of 46 pea genotypes were screened against the E. pisi isolate- Ep01 in greenhouse conditions to identify resistant genotypes. Disease reaction and genotyping were carried out to test for resistance/ susceptibility through gene-specific sequence characterized amplified region (SCAR) markers. The presence of the resistance alleles in the control pea genotypes- JI2302 for er1 gene, JI2480 for er2 gene and P660–4 for Er3 was confirmed through their respective gene-specific markers. The pea genotype- Arkel served as a negative control. Screening of the pea germplasm revealed 3 genotypes as highly resistant, 6 genotypes as resistant while 10 genotypes were moderately resistant, 13 genotypes were moderately susceptible, 9 genotypes were susceptible and 5 were highly susceptible. The molecular marker for er1 resistance gene Sc-OPE-161600 was found to be segregating in most of the pea genotypes except in the susceptible control- Arkel. The marker- ScX17_1400 for er2 gene was found to be in homozygous condition in the resistant pea genotypes while the marker associated with Er3 resistance, SCW4637, was found to be in heterozygous condition in majority of the pea genotypes except in the resistant control genotype- P660–4.

Abstract Image

利用分子标记通过疾病表型和基因分型评价豌豆种质的白粉病抗性
豌豆丹毒是引起豌豆白粉菌感染的主要病原之一。到目前为止,已经报道了由两个隐性(er1和er2)和一个显性(Er3)组成的抗性基因赋予豌豆对白粉菌的抗性。在温室条件下对豌豆E.pisi分离株-Ep01筛选了一组46种豌豆基因型,以鉴定抗性基因型。通过基因特异性序列特征扩增区(SCAR)标记进行疾病反应和基因分型以检测耐药性/易感性。对照豌豆基因型中存在抗性等位基因——er1基因的JI2302、er2基因的JI2480和Er3基因的P660–4——通过它们各自的基因特异性标记得到了证实。豌豆基因型Arkel作为阴性对照。豌豆种质资源筛选结果表明,3个基因型为高抗性,6个基因型抗性,10个基因型中度抗性,13个基因型中等敏感,9个基因型敏感,5个基因型高度敏感。er1抗性基因Sc-OPE-161600的分子标记在除敏感对照Arkel外的大多数豌豆基因型中都是分离的。er2基因的标记-ScX17_1400在抗性豌豆基因型中被发现处于纯合状态,而与Er3抗性相关的标记SCW4637在除抗性对照基因型P660-4外的大多数豌豆基因型都处于杂合状态。
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来源期刊
Plant Gene
Plant Gene Agricultural and Biological Sciences-Plant Science
CiteScore
4.50
自引率
0.00%
发文量
42
审稿时长
51 days
期刊介绍: Plant Gene publishes papers that focus on the regulation, expression, function and evolution of genes in plants, algae and other photosynthesizing organisms (e.g., cyanobacteria), and plant-associated microorganisms. Plant Gene strives to be a diverse plant journal and topics in multiple fields will be considered for publication. Although not limited to the following, some general topics include: Gene discovery and characterization, Gene regulation in response to environmental stress (e.g., salinity, drought, etc.), Genetic effects of transposable elements, Genetic control of secondary metabolic pathways and metabolic enzymes. Herbal Medicine - regulation and medicinal properties of plant products, Plant hormonal signaling, Plant evolutionary genetics, molecular evolution, population genetics, and phylogenetics, Profiling of plant gene expression and genetic variation, Plant-microbe interactions (e.g., influence of endophytes on gene expression; horizontal gene transfer studies; etc.), Agricultural genetics - biotechnology and crop improvement.
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