Changes in human sperm motility and DNA fragmentation index after incubation at different temperatures following density gradient centrifugation and swim-up procedures

IF 0.7 4区医学 Q4 OBSTETRICS & GYNECOLOGY

Reproductive and Developmental Medicine Pub Date : 2022-09-21 DOI:10.1097/RD9.0000000000000039

Yan-Nan Yang, Ling Wang, Yubao Liu, Yun-jing Xue, Chen-Chen Liu, Fei Shi, Xue Dai, R. Chian

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引用次数: 1

Abstract

Objective: The purpose of this study was to evaluate the sperm motility and DNA integrity at different temperatures to analyze whether the sperms are suitable on the second day for insemination of in vitro matured oocytes by intra-cytoplasmic sperm injection (ICSI) following density gradient centrifugation (DGC) and swim-up (SU) procedures. Methods: Semen samples were collected from 30 outpatients who visited the Center for Reproductive Medicine for semen analyses. Following sperm selection by DGC and SU procedures, the liquified semen samples were divided into three groups and incubated at 4, 25, and 37°C, respectively. Following incubation for 24, 48, and 72 hours, the sperm motility and sperm DNA fragmentation index (DFI) were analyzed. Results: Following the combination of DGC and SU procedures, the sperm motility (91.8% ± 8.6% vs. 50.8% ± 13.1%) and DFI (5.1% ± 7.9% vs. 13.0% ± 11.6%) were significantly improved (P <0.01) compared to those without any treatment. The sperm motility of the 3 groups significantly declined (P <0.05) post-incubation compared to that of the groups prior incubation. However, sperm motility significantly increased (76.9% ± 10.4%) (P <0.05) at 25°C compared to that of the other 2 groups (53.5% ± 11.0% and 47.6% ± 10.2%). Sperm DFI significantly increased (P <0.05) at 37°C following incubation for 24 and 72 hours in comparison to that of the other 2 groups. However, the sperm DFI did not significantly increase when the sperm samples were incubated at 4 (5.7% ± 5.9%) and 25°C (6.8% ± 5.6%) for 24 hours compared to that before incubation (5.1% ± 7.9%). Conclusions: These results indicate that the sperm quality, in terms of motility and DFI, can be efficiently improved by DGC in combination with SU. Following which, the sperm samples can be incubated at 25°C and be used on the second day for insemination of in vitro matured oocytes by ICSI.

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在密度梯度离心和游泳程序下，不同温度孵育后人类精子活力和DNA断裂指数的变化

目的：本研究旨在评估不同温度下精子的活力和DNA完整性，以分析精子是否适合在第二天通过密度梯度离心（DGC）和向上游动（SU）程序进行细胞质内精子注射（ICSI）受精体外成熟卵母细胞。方法：从生殖医学中心就诊的30名门诊患者中采集精液样本进行精液分析。通过DGC和SU程序选择精子后，将液化精液样本分为三组，分别在4、25和37°C下孵育。孵育24、48和72小时后，分析精子活力和精子DNA断裂指数（DFI）。结果：DGC和SU联合治疗后，精子活力（91.8%±8.6%对50.8%±13.1%）和DFI（5.1%±7.9%对13.0%±11.6%）与未经任何治疗的相比有显著改善（P<0.01）。与孵育前相比，孵育后3组的精子活力显著下降（P<0.05）。然而，与其他两组（53.5%±11.0%和47.6%±10.2%）相比，25°C时精子活力显著增加（76.9%±10.4%）（P<0.05）。与其他两个组相比，37°C孵育24和72小时后精子DFI显著增加（P<0.05）。然而，当精子样品在4（5.7%±5.9%）和25°C（6.8%±5.6%）下孵育24小时时，与孵育前（5.1%±7.9%）相比，精子DFI没有显著增加。结论：这些结果表明，DGC与SU联合使用可以有效地提高精子的运动性和DFI质量，精子样品可以在25°C下孵育，并在第二天通过ICSI用于体外成熟卵母细胞的受精。

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