Phenotypic Antimicrobial Resistance Patterns in Salmonella typhimurium and Enteritidis Strains Isolated from Human, Food, and Environmental Samples of Broiler Meat Production Chain in Punjab

Q2 Agricultural and Biological Sciences

International Journal of Agriculture and Biology Pub Date : 2021-08-01 DOI:10.17957/ijab/15.1833

Shakera Sadiq

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引用次数: 0

Abstract

The emergence of antimicrobial resistance is a growing public health issue worldwide due to extensive use in agriculture, food, and veterinary medicine. The rate of antimicrobial resistance varies with different antibiotics and serotypes but Salmonella enterica serovar Enteritidis is one of the most widespread serotypes which is comparatively more susceptible to antimicrobial resistance followed by S. Typhimurium. A total of 71 Salmonella strains (S. Typhimurium, n=45; S. Enteritidis, n=26) isolated from humans, food and the environment were used in the study. Both isolates were confirmed through PCR by targeting their specific spy and sdf genes respectively. Kirby-Bauer disc diffusion method was used to determine antimicrobial susceptibility against 10 antibiotics used. An exponentially high level of resistance was found in S. Typhimurium strains. The highest level of resistance was found against amoxicillin (97.78%) followed by tetracycline (95.56%), gentamicin (93.33%), trimethoprim (86.67%), streptomycin (84.44%), nalidixic acid (77.78%), sulphafurazole (64.44%), ampicillin (62.22%), chloramphenicol (46.67%) and least resistance was found against ciprofloxacin (31.11%). Four penta MDR (ACSSuT) and two tetra MDR (ASSuT) resistance patterns were found S. Typhimurium strains. In S. Enteritidis strains, a high level of resistance was found against sulphonamides and streptomycin (92.31%) with the least resistance against ciprofloxacin (11.54%). Two penta MDR (ACSSuT) and six tetra MDR (ASSuT) resistance patterns were found. The presence of high antimicrobial resistance in zoonotic S. Typhimurium and Enteritidis in the broiler meat production chain is alarming. Immediate action and appropriate measures are required to control over the counter and irrational use of antibiotics both in poultry and humans. © 2021 Friends Science Publishers

查看原文本刊更多论文

旁遮普省肉鸡肉生产链人类、食品和环境样本中鼠伤寒沙门氏菌和肠炎菌株的表型耐药性模式

由于在农业、食品和兽医中的广泛应用，抗微生物耐药性的出现是世界范围内日益严重的公共卫生问题。抗微生物耐药性的发生率因不同的抗生素和血清型而异，但肠炎沙门氏菌血清型是最广泛的血清型之一，相对而言更容易产生抗微生物耐药性，其次是鼠伤寒沙门氏菌。本研究共使用了71株从人类、食物和环境中分离的沙门氏菌（鼠伤寒沙门氏菌，n=45；肠炎沙门氏杆菌，n=26）。这两个分离株分别通过特异性spy和sdf基因的聚合酶链式反应得到证实。采用Kirby-Bauer纸片扩散法测定对所用10种抗生素的耐药性。在鼠伤寒沙门氏菌菌株中发现了指数级高水平的抗性。对阿莫西林的耐药性最高（97.78%），其次是四环素（95.56%）、庆大霉素（93.33%）、甲氧苄啶（86.67%）、链霉素（84.44%）、萘啶酸（77.78%）、磺脲唑（64.44%）、氨苄青霉素（62.22%），对环丙沙星的耐药率最低（31.11%），对氯霉素的耐药率为46.67%。Enteritidis菌株对磺酰胺类和链霉素的耐药性较高（92.31%），对环丙沙星的耐药性最低（11.54%）。肉鸡生产链中人畜共患鼠伤寒沙门氏菌和肠炎杆菌的高抗微生物耐药性令人担忧。需要立即采取行动并采取适当措施，控制家禽和人类对抗生素的反作用和不合理使用。©2021 Friends Science出版社

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来源期刊

International Journal of Agriculture and Biology AGRICULTURE, MULTIDISCIPLINARY-

CiteScore

1.70

自引率

0.00%

发文量

审稿时长

5 months

期刊介绍： Information not localized