Pseudomonas sp., Moraxella sp., Vibrio sp. Dari Sedimen Mangrove Sebagai Antibakteri Terhadap Escherichia coli, Staphylococcus aureus dan Salmonella thypi

IF 0.5 4区地球科学 Q3 Earth and Planetary Sciences

Journal of Marine Research Pub Date : 2022-07-23 DOI:10.14710/jmr.v11i3.35037

Sylvia Sari Indah Dongoran, S. Subagiyo, W. A. Setyati

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引用次数: 0

Abstract

Resistensi bakteri merupakan isu global yang sangat penting. Infeksi penyakit disebabkan oleh resistensi bakteri. E. coli, S. aureus dan S. thypi merupakan bakteri patogen yang menyebabkan infeksi. Pengendalian resistensi bakteri dapat dilakukan dengan eksplorasi sumber senyawa baru. Bakteri sedimen mangrove berpotensi sebagai antibakteri terhadap E. coli, S. aureus dan S. thypi. Tujuan dari penelitian ini dilakukan untuk memperoleh jenis bakteri sedimen mangrove yang memiliki aktivitas antibakteri terhadap E. coli, S. aureus dan S. thypi. Penelitian terdiri dari beberapa tahapan dimulai dari isolasi, purifikasi, uji aktivitas antibakteri dan uji biokimia. Hasil isolasi yang diperoleh berjumlah 33 isolat, 3 diantaranya memiliki aktivitas antibakteri terhadap E. coli, S. aureus dan S. thypi. Berdasarkan hasil yang didapatkan, Aktivitas antibakteri SS.2 ISP tergolong kuat terhadap E. coli dan tergolong sedang terhadap S. aureus dan S. thypi. Aktivitas antibakteri SS.4 ISP tergolong sedang terhadap E. coli, S. aureus dan S. thypi sedangkan SK.1 MA tergolong sedang terhadap E. coli dan S. aureus dan tergolong lemah terhadap S. thypi. Berdasarkan identifikasi biokimia isolat SS.2 ISP, SS.4 ISP dan SK.1 MA teridentifikasi sebagai genus Pseudomonas sp., Moraxella sp. dan Vibrio sp.. Bacterial resistance is a very important global issue. Infectious diseases are caused by bacterial resistance. E. coli, S. aureus and S. thypi are pathogenic bacteria that cause infection. Control of bacterial resistance can be done by exploring new sources of compounds. Mangrove sediment bacteria have the potential as antibacterial against E. coli, S. aureus and S. thypi. The purpose of this study was to obtain types of mangrove sediment bacteria that have antibacterial activity against E. coli, S. aureus and S. thypi. The research consisted of several stages starting from isolation, purification, antibacterial activity test and biochemical test. The isolation results obtained were 33 isolates, 3 of which had antibacterial activity against E. coli, S. aureus and S. thypi. Based on the results obtained, the antibacterial activity of SS.2 ISP was strong against E. coli and moderate against S. aureus and S. thypi. The antibacterial activity of SS.4 ISP was moderate against E. coli, S. aureus and S. thypi, while SK.1 MA was moderate against E. coli and S. aureus and weak against S. thypi. Based on the biochemical identification of isolates SS.2 ISP, SS.4 ISP and SK.1 MA were identified as the genus Pseudomonas sp., Moraxella sp. and Vibrio sp.

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Sedimen红树林假单胞菌属、莫拉克菌属、弧菌属抗大肠杆菌、金黄色葡萄球菌和百里香沙门氏菌

抗菌耐药性是一个非常重要的全球问题。由抗菌引起的疾病感染。大肠杆菌，大肠杆菌和大肠杆菌是导致感染的病原体。可以通过探索新的化合物来源来进行抗菌防御。mangrove细菌是大肠杆菌、大肠杆菌和thypi的潜在抗菌。这项研究的目的是获得具有抗菌性大肠杆菌、大肠杆菌和thypi抗菌活性的mangrove细菌。研究包括几个阶段，从隔离、净化、抗菌活性试验和生物化学试验开始。我们得到了33种分离物，其中3种具有抗菌活性。根据所取得的结果，SS.2 ISP的抗菌活性为大肠杆菌强，而S. aureus和S. thypi。SS.4个ISP属于E.大肠杆菌、S. aureus和S. ni，而SK.1 MA属于E. coli和S. aureus，而y . aureus和S. aureus都属于S. thypi。根据生物化学鉴定，党卫军是隔离的。2个ISP, 4个ISP和SK。1个MA被确认为伪单胞菌属、Moraxella sp和弧菌属。细菌抵抗是一个非常重要的全球问题。传染病是由细菌抵抗引起的。大肠杆菌，S. aureus和S. thypi是导致感染的病毒性细菌。这种细菌阻力可以由联合探索的新资源来实现。Mangrove沉积细菌具有潜在的抗大肠杆菌、S. aureus和S. thypi。这项研究的目的是清除一种抗杆菌、大肠杆菌、S. aureus和S. thypi的传染性细菌。研究发现，从孤立、净化、抗细菌反应测试和生物化学测试开始的几个阶段。消化道的结果是33个异位，其中3个有抗杆菌作用于大肠杆菌、S. aureus和S. thypi。基于受感染的替代品，SS.2 ISP强烈反对大肠杆菌和温和的thypi。SS.4个ISP对大肠杆菌、S. aureus和S. thypi都有温和的抑制，而SK.1 MA对大肠杆菌和S. aureus和S. aureus和weak against S. thypi。基于党卫军的生物化学标识。2个ISP, 4个ISP和SK。1个MA被认为是假单胞菌属、Moraxella sp和Vibrio sp。

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来源期刊

Journal of Marine Research 地学-海洋学

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0.00%

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审稿时长

3 months

期刊介绍： The Journal of Marine Research publishes peer-reviewed research articles covering a broad array of topics in physical, biological and chemical oceanography. Articles that deal with processes, as well as those that report significant observations, are welcome. In the area of biology, studies involving coupling between ecological and physical processes are preferred over those that report systematics. Authors benefit from thorough reviews of their manuscripts, where an attempt is made to maximize clarity. The time between submission and publication is kept to a minimum; there is no page charge.