A New SNPs at 3’UTR Region of calpain 1 gene and its association with growth and meat quality traits in beef cattle

IF 0.3 Q4 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Journal of the Indonesian Tropical Animal Agriculture Pub Date : 2021-08-29 DOI:10.14710/jitaa.47.1.17-28

D. Dairoh, J. Jakaria, M. Ulum, C. Sumantri

{"title":"A New SNPs at 3’UTR Region of calpain 1 gene and its association with growth and meat quality traits in beef cattle","authors":"D. Dairoh, J. Jakaria, M. Ulum, C. Sumantri","doi":"10.14710/jitaa.47.1.17-28","DOIUrl":null,"url":null,"abstract":"The objective of this study was to evaluate single nucleotide polymorphisms (SNP) in the 3'UTR region of CAPN1 gene by direct sequencing technique in beef cattle and its influence on growth and meat quality of the contrast mode ultrasound image in Bali cattle. The numbers of beef cattle used were Bali cattle (n=52 heads) which compared to purebred Belgian Blue (n=30 heads), Limousine (n=14 heads), Wagyu (n=7 heads), and PO (n=10 heads). Genetic diversity data were obtained from calculations by PopGen 1.32 software. Ultrasound imaging of the thoracic longissimus dorsi muscle in Bali cattle was carried out between the 12 − 13 ribs, and the sonogram was analyzed by Image-J NIH software. The result shown that 3’UTR of CAPN1 gene was found in six discovery SNPs that polymorphic in Bali cattle, they were g.15284 C>T, g.15347 T>G, g.15525 G>A, g.15853 G>A, g.15905 G>A, g.15915 G>A and Indel mutation was polymorphic in Bali cattle, Belgian Blue, Limousine, and PO. There was no association between these SNPs and growth traits. However, SNP g.15525 G>A was significantly associated (P<0.05) with a backfat thickness (BFT) in Bali cattle. In conclusion, the CAPN1 gene in Bali cattle is a candidate for Marker Assisted Selection (MAS) related to meat quality.","PeriodicalId":17297,"journal":{"name":"Journal of the Indonesian Tropical Animal Agriculture","volume":" ","pages":""},"PeriodicalIF":0.3000,"publicationDate":"2021-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of the Indonesian Tropical Animal Agriculture","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.14710/jitaa.47.1.17-28","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"AGRICULTURE, DAIRY & ANIMAL SCIENCE","Score":null,"Total":0}

引用次数: 0

Abstract

The objective of this study was to evaluate single nucleotide polymorphisms (SNP) in the 3'UTR region of CAPN1 gene by direct sequencing technique in beef cattle and its influence on growth and meat quality of the contrast mode ultrasound image in Bali cattle. The numbers of beef cattle used were Bali cattle (n=52 heads) which compared to purebred Belgian Blue (n=30 heads), Limousine (n=14 heads), Wagyu (n=7 heads), and PO (n=10 heads). Genetic diversity data were obtained from calculations by PopGen 1.32 software. Ultrasound imaging of the thoracic longissimus dorsi muscle in Bali cattle was carried out between the 12 − 13 ribs, and the sonogram was analyzed by Image-J NIH software. The result shown that 3’UTR of CAPN1 gene was found in six discovery SNPs that polymorphic in Bali cattle, they were g.15284 C>T, g.15347 T>G, g.15525 G>A, g.15853 G>A, g.15905 G>A, g.15915 G>A and Indel mutation was polymorphic in Bali cattle, Belgian Blue, Limousine, and PO. There was no association between these SNPs and growth traits. However, SNP g.15525 G>A was significantly associated (P<0.05) with a backfat thickness (BFT) in Bali cattle. In conclusion, the CAPN1 gene in Bali cattle is a candidate for Marker Assisted Selection (MAS) related to meat quality.

查看原文本刊更多论文

肉牛钙蛋白酶1基因3'UTR区新snp及其与生长和肉质性状的关系

本研究的目的是通过直接测序技术评估肉牛CAPN1基因3’UTR区的单核苷酸多态性（SNP）及其对巴厘牛生长和肉质的影响。使用的肉牛数量为巴厘牛（n=52头），与纯种比利时蓝（n=30头）、利穆赞（n=14头）、和牛（n=7头）和PO（n=10头）相比。遗传多样性数据是通过PopGen 1.32软件计算获得的。对巴厘牛胸背最长肌12～13根肋骨间进行了超声成像，并用Image-J NIH软件对其声像图进行了分析。结果表明，在6个巴厘牛发现的多态性SNPs中发现CAPN1基因的3’UTR，分别为g.15284C>T、g.15347T>g、g.15525G>A、g.15853G>A，g.15905G>A和g.15915G>A。这些SNPs与生长性状之间没有关联。然而，巴厘牛的SNP g.15525G>A与背涂层厚度（BFT）显著相关（P<0.05）。总之，巴厘牛的CAPN1基因是与肉质相关的标记辅助选择（MAS）的候选者。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Journal of the Indonesian Tropical Animal Agriculture AGRICULTURE, DAIRY & ANIMAL SCIENCE-

CiteScore

1.10

自引率

0.00%

发文量

审稿时长

8 weeks

期刊介绍： Journal of the Indonesian Tropical Animal Agriculture (JITAA) is a double blind peer-reviewed publication devoted to disseminate all information contributing to the understanding and development of animal agriculture in the tropics by publication of original research papers. The journal covers all aspect relating to Animal Agriculture, including: -Animal breeding and genetics -Animal feeding and nutrition -Animal reproduction -Animal biotechnology -Animal physiology -Animal production and technology -Animal products technology -Animal management and economics -Animal products processing and animal by-products -Animal microbiology -Livestock farming systems -Other related topics in relation to animal science. The topics of research are not only on Indonesian tropical animal agriculture, but also on other tropical regions of the world.