Benefits of tributyrin on growth performance, gastrointestinal tract development, ruminal bacteria and volatile fatty acid formation of weaned Small-Tailed Han lambs

IF 6.3

Animal Nutrition Pub Date : 2023-08-30 DOI:10.1016/j.aninu.2023.08.006

Zhiwei Li , Xueer Wang , Wei Wang , Ran An , Yaxin Wang , Qingchang Ren , Jingjing Xuan

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引用次数: 1

Abstract

This study aimed to determine the effects of tributyrin on growth performance, gastrointestinal tract development, ruminal bacteria and volatile fatty acid (VFA) formation. Thirty healthy weaned Small-Tailed Han female lambs at 3 months old with BW 27.5 ± 4.1 kg (mean ± SD) were randomly assigned to five groups of six lambs each, and each group received tributyrin at 0, 0.5, 1.0, 2.0 and 4.0 g/kg in feed. Weights were measured before the start and end of the study. After 15 d adaptation, DMI, feed, faeces and urine were recorded every week. Lambs were sacrificed at d 75. Compared to lambs fed no tributyrin, lambs fed 4.0 g/kg tributyrin had higher average daily BW gain (P = 0.04) and DMI (P < 0.01). Tributyrin reduced nitrogen (P < 0.01), Ca (P < 0.01) and P (P < 0.01) losses derived from faeces and urine. The mostly important, tributyrin increased dorsal sac thickness (P < 0.01), papillae length (P = 0.04) and width (P < 0.01), ventral sac papillae length (P < 0.01) and width (P < 0.01), caudodorsal blind sac thickness (P = 0.02), papillae length (P < 0.01) and width (P < 0.01). Furthermore, tributyrin increased thicknesses of both the duodenum (P < 0.01) and ileum (P = 0.01), and villus heights of the duodenum (P = 0.01), ileum (P < 0.01), jejunum (P < 0.01) and caecum (P = 0.02), but tributyrin decreased duodenal (P < 0.01) and caecal crypt depths (P < 0.01). Tributyrin reduced rumen pH (P < 0.01) while promoting total VFA concentration (P < 0.01). Tributyrin improved the structure of rumen bacteria by enhancing Clostridium (P = 0.04), Butyrivibrio (P < 0.01), Streptococcus (P = 0.04), Prevotella (P = 0.04), Ruminobacter (P = 0.02) and Fibrobacter (P = 0.03). In conclusion, tributyrin could stimulate gastrointestinal tract development by enhancing colonization of rumen VFA-producing bacteria, and dietary supplementation of tributyrin at 4.0 g/kg of DM was recommended for the weaned lambs.

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三丁酸甘油酯对断奶小尾寒羔羊生长性能、胃肠道发育、瘤胃细菌和挥发性脂肪酸形成的影响

本试验旨在研究三丁酸甘油酯对生长性能、胃肠道发育、瘤胃细菌和挥发性脂肪酸(VFA)形成的影响。选取30只3月龄、体重27.5±4.1 kg (mean±SD)、健康的断奶小尾汉母羊，随机分为5组，每组6只，每组分别饲喂0、0.5、1.0、2.0和4.0 g/kg的三丁酸甘油酯饲料。在研究开始和结束前测量了体重。适应15 d后，每周记录DMI、饲料、粪便和尿液。羔羊在75岁时被宰杀。与不饲喂三丁酸甘油酯的羔羊相比，饲喂4.0 g/kg三丁酸甘油酯的羔羊平均日增重(P = 0.04)和DMI (P <0.01)。三丁酸甘油酯还原氮(P <0.01)， Ca (P <0.01)和P (P <0.01)由粪便和尿液引起的损失。最重要的是，三丁酸甘油酯增加了背囊厚度(P <0.01)，乳头长度(P = 0.04)和宽度(P <0.01)，腹侧囊乳头长度(P <0.01)和宽度(P <0.01)，尾背盲囊厚度(P = 0.02)，乳头长度(P <0.01)和宽度(P <0.01)。此外，三丁酸甘油酯增加了十二指肠(P <0.01)、回肠(P = 0.01)、十二指肠绒毛高度(P = 0.01)、回肠(P <0.01)，空肠(P <0.01)和盲肠(P = 0.02)，但三丁酸甘油酯降低了十二指肠(P <0.01)和盲肠隐窝深度(P <0.01)。三丁酸甘油酯降低瘤胃pH (P <0.01)，同时提高总VFA浓度(P <0.01)。三丁酸甘油酯通过增强梭状芽胞杆菌(P = 0.04)、丁酸弧菌(P <0.01)、链球菌(P = 0.04)、普雷沃氏菌(P = 0.04)、瘤胃杆菌(P = 0.02)和纤维杆菌(P = 0.03)。综上所述，三丁酸甘油酯可以通过促进瘤胃vfa产生菌的定植来促进胃肠道发育，建议在断奶羔羊饲粮中添加4.0 g/kg DM的三丁酸甘油酯。

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来源期刊

Animal Nutrition Animal Science and Zoology

CiteScore

9.70

自引率

0.00%

发文量

542

审稿时长

65 days

期刊介绍： Animal Nutrition encompasses the full gamut of animal nutritional sciences and reviews including, but not limited to, fundamental aspects of animal nutrition such as nutritional requirements, metabolic studies, body composition, energetics, immunology, neuroscience, microbiology, genetics and molecular and cell biology related to primarily to the nutrition of farm animals and aquatic species. More applied aspects of animal nutrition, such as the evaluation of novel ingredients, feed additives and feed safety will also be considered but it is expected that such studies will have a strong nutritional focus. Animal Nutrition is indexed in SCIE, PubMed Central, Scopus, DOAJ, etc.