Characterization and Extraction of Bioactive Compounds in Ethanolic Extract of Moringa concanensis, Nimmo Leaf, Using Microwave-assisted and Soxhlet Techniques

Abstract

The extraction of Moringa concanensis (Nimmo leaf) was done in ethanol using Soxhlet and microwave-assisted extraction (MAE) techniques to obtain bioactive compounds. The standard techniques were used to carry out phytochemical analysis of ethanolic extract. The analysis confirmed the presence of flavonoids, phenols, steroids, alkaloids, terpenoids, glycosides, tannins, and saponins, and also the absence of anthraquinones. Moreover, the extract was evaluated for antioxidant activities using DPPH and ABTS assays. The IC50 value of the extract was also determined. A clear correlation between the total phenolic content, total flavonoid content, and antioxidant activity of the leaf extracts was obtained. Conclusion: This study suggests that a high amount of phenolic compounds could be obtained from Moringa concanensis (Nimmo leaf) by using the microwave-assisted extraction technique, which may be responsible for its antioxidant activity.