Nano-selenium impacts on growth performance, digestive enzymes, antioxidant, immune resistance and histopathological scores of Nile tilapia, Oreochromis niloticus against Aspergillus flavus infection

IF 2.2 3区农林科学 Q2 FISHERIES

Aquaculture International Pub Date : 2023-08-30 DOI:10.1007/s10499-023-01230-4

El-Sayed Hemdan Eissa, Walaa K. Bazina, Yasmin M. Abd El-Aziz, Nadia A. Abd Elghany, Wesam A. Tawfik, Monga I. Mossa, Ola Hasan Abd El Megeed, Nadia N. B. Abd El-Hamed, Adel F. El-Saeed, Ehab El-Haroun, Simon J. Davies, Oliver J. Hasimuna, Moaheda E. H. Eissa, Hala S. Khalil

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引用次数: 0

Abstract

The present study examined the impacts of varying amounts of selenium nanoparticles (Se-NPs) as a natural antioxidant and metabolic regulator on growth performance, antioxidant capacity, digestive enzymes and immune resistance of Oreochromis niloticus challenged to Aspergillus flavus infection. For 60 days, fish were fed the experimental diet of 30% crude protein up to apparent satiation three times a day. Se-NPs were added to the control diet at different levels of 0.0 (control), 0.5 (T1), 1.0 (T2) and 1.5 (T3) mg/kg diet to fed monosex Nile tilapia fingerlings with average initial weight (4.5 ± 0.5 g). Fish were randomly distributed in 12 tanks 100 L at a density of 20 fish per tank after 2-week acclimation to represent four treatments in triplicates. The results indicated that the growth indices, feed efficiency and survival rate were significantly enhanced (P < 0.05) by incorporating Se-NPs up to 1 mg/kg diet. Furthermore, the haemato-biochemical parameters, digestive enzymes activity and antioxidant capacity of the fish were significantly improved (P < 0.05) at T2. When the fish were challenged with Aspergillus flavus, a decreasing mortality rate was observed, which clearly shows that selenium nanoparticles boosted the fish’s immune response at T2. The intestinal morphology as villus length, villus width, muscular fibres layer thickness and absorption intestinal zone after challenged with A. flavus infection were considerably enhanced (P < 0.05) by incorporating all Se-NP levels. Histopathological score significantly improved (P < 0.05) for the hepatopancreatic, intestinal, gills and muscle tissues at T2. The present study concludes that selenium nanoparticles up to 1.0 mg/kg diet can be efficiently used in tilapia feed to help boost fish production, immune system response and histopathological parameters.

查看原文本刊更多论文

纳米硒对尼罗罗非鱼(Oreochromis niloticus)生长性能、消化酶、抗氧化、免疫抵抗力和抗黄曲霉组织病理学评分的影响

本研究考察了作为天然抗氧化剂和代谢调节剂的不同量纳米硒粒子（Se-NPs）对受黄曲霉菌感染的裸鲤的生长性能、抗氧化能力、消化酶和免疫抵抗力的影响。在 60 天的时间里，每天三次给鱼喂食含 30% 粗蛋白的实验饲料，直至明显饱腹。将 Se-NPs 按 0.0（对照组）、0.5（T1）、1.0（T2）和 1.5（T3）毫克/千克的不同水平添加到对照组日粮中，喂养平均初始体重（4.5 ± 0.5 克）的单性尼罗罗非鱼幼苗。鱼在适应 2 周后随机分布在 12 个 100 L 的水槽中，密度为每槽 20 条鱼，以三重复代表四种处理。结果表明，添加 Se-NPs 至 1 mg/kg 日粮可显著提高生长指数、饲料效率和存活率（P < 0.05）。此外，在 T2 期，鱼的血液生化指标、消化酶活性和抗氧化能力也有明显改善（P < 0.05）。当鱼类受到黄曲霉挑战时，死亡率下降，这清楚地表明纳米硒颗粒在 T2 阶段提高了鱼类的免疫反应。加入各种水平的 Se-NP 后，黄曲霉菌感染后的肠道形态，如绒毛长度、绒毛宽度、肌纤维层厚度和肠道吸收区均显著增强（P < 0.05）。在 T2 期，肝胰腺、肠道、鳃和肌肉组织的组织病理学评分明显提高（P < 0.05）。本研究得出结论，硒纳米颗粒（最高达 1.0 毫克/千克日粮）可有效地用于罗非鱼饲料，帮助提高鱼类产量、免疫系统反应和组织病理学参数。

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来源期刊

Aquaculture International 农林科学-渔业

CiteScore

5.10

自引率

6.90%

发文量

204

审稿时长

1.0 months

期刊介绍： Aquaculture International is an international journal publishing original research papers, short communications, technical notes and review papers on all aspects of aquaculture. The Journal covers topics such as the biology, physiology, pathology and genetics of cultured fish, crustaceans, molluscs and plants, especially new species; water quality of supply systems, fluctuations in water quality within farms and the environmental impacts of aquacultural operations; nutrition, feeding and stocking practices, especially as they affect the health and growth rates of cultured species; sustainable production techniques; bioengineering studies on the design and management of offshore and land-based systems; the improvement of quality and marketing of farmed products; sociological and societal impacts of aquaculture, and more. This is the official Journal of the European Aquaculture Society.