Antioxidant and tyrosinase inhibitory activities of A. sicula L. aqueous extract

Q4 Pharmacology, Toxicology and Pharmaceutics
K. Loucif, Fatima Benchikh, Hassiba Benabdallah, C. Bensouici, Smain Amira
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引用次数: 0

Abstract

Overexpression of tyrosinase in humans causes an increase in melanin production in the skin, which can result in hyperpigmentation consequences such as freckles, melasma, age spots, and melanoma. Free radicals also play a significant role in the increase of the biosynthesis of melanin. Tyrosinase inhibitors capable of inhibiting the biosynthesis of melanin are used currently in various hyperpigmentation and cosmetic agents to control the formation of freckles. Several synthetic tyrosinase inhibitors have been associated with several serious side effects. Also, synthetic antioxidants had many toxicological side effects including carcinogenicity. There is an increasing interest in the search for natural tyrosinase inhibitors and antioxidant agents. The objective of this study is to evaluate total polyphenol and flavonoid contents as well as examine the antioxidative and tyrosinase inhibitory effects of A. sicula L. aqueous extract. Antioxidant activities evaluated using superoxide radical scavenging and reducing power methods. tyrosinase inhibitory assay was used to determine anti-hyperpigmentation. The results showed that this extract was rich in total polyphenols (58.01±1.18 micrograms of gallic acid equivalents per milligrams of extract) and flavonoids (17.91±1.81 micrograms quercetin equivalents per milligram of extract). A. sicula L. aqueous extract was capable of scavenging free radicals (IC50 =11.87±0.13 μg/mL) and acts as a strong reducing agent (A 0.5= 6.37±0.42µg/mL). A. sicula L. had a potent tyrosinase inhibitory potential (IC50= 12.63±1.15 μg/mL), which was higher compared to kojic acid as standard (IC50= 25,23±0,78 μg/mL, P <0.001). These results support that A. sicula L. could be a new source of antioxidant and cosmetic use. Further studies focusing on the isolation and characterization of active principles of antioxidant and tyrosinase inhibitory activities are needed.
仙人掌水提取物的抗氧化和酪氨酸酶抑制活性
人体中酪氨酸酶的过度表达会导致皮肤中黑色素的产生增加,从而导致色素沉着过度,如雀斑、黄褐斑、老年斑和黑色素瘤。自由基在增加黑色素的生物合成中也起着重要作用。酪氨酸酶抑制剂能够抑制黑色素的生物合成,目前被用于各种色素沉着和化妆品中,以控制雀斑的形成。几种合成酪氨酸酶抑制剂与几种严重的副作用有关。此外,合成抗氧化剂有许多毒理学副作用,包括致癌性。人们对寻找天然酪氨酸酶抑制剂和抗氧化剂越来越感兴趣。本研究的目的是评价花楸水提物的总多酚和类黄酮含量,并考察花楸水提物的抗氧化和酪氨酸酶抑制作用。用超氧自由基清除和还原力法评价抗氧化活性。酪氨酸酶抑制法测定抗色素沉着。结果表明,该提取物含有丰富的总多酚(58.01±1.18微克没食子酸当量/毫克)和总黄酮(17.91±1.81微克槲皮素当量/毫克)。花楸水提物具有较强的自由基清除能力(IC50 =11.87±0.13 μg/mL)和强还原剂(IC50 = 6.37±0.42 μg/mL)。对酪氨酸酶有较强的抑制作用(IC50= 12.63±1.15 μg/mL),高于对照品曲酸(IC50= 25、23±0.78 μg/mL, P <0.001)。这些结果支持了花楸属植物可能成为抗氧化和化妆品的新来源。需要进一步研究其抗氧化活性和酪氨酸酶抑制活性的分离和表征。
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来源期刊
Current Enzyme Inhibition
Current Enzyme Inhibition Pharmacology, Toxicology and Pharmaceutics-Drug Discovery
CiteScore
1.30
自引率
0.00%
发文量
30
期刊介绍: Current Enzyme Inhibition aims to publish all the latest and outstanding developments in enzyme inhibition studies with regards to the mechanisms of inhibitory processes of enzymes, recognition of active sites, and the discovery of agonists and antagonists, leading to the design and development of new drugs of significant therapeutic value. Each issue contains a series of timely, in-depth reviews written by leaders in the field, covering a range of enzymes that can be exploited for drug development. Current Enzyme Inhibition is an essential journal for every pharmaceutical and medicinal chemist who wishes to have up-to-date knowledge about each and every development in the study of enzyme inhibition.
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