Application of Pigments to Squirrel Legs at Various Stages of the Plastination Process

Q4 Medicine
R. Lewis, J. A. Huggins, R. A. Wamble, M. Bolyard
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引用次数: 0

Abstract

Department of Biology, Union University, Jackson, TN 38305 USA. ABSTRACT: Plastinated tissues have gained popularity due to their structural integrity and the ease in handling and storing. Specimens can lose natural (or added) color throughout the plastination process, and so one objective of coloration is to restore natural color. A second objective of this study was to measure color change, both objectively and by subjective visualization of coloration. Coloration was investigated using 3 pigments added at 4 defined points during the 5-stage plastination process, using squirrel leg muscle samples. Each of the 12 experimental groups, and an untreated sample, were analyzed by measuring the Euclidean distance and CIE2000 E values. Statistical analysis indicated that none of the coloration treatments restored natural coloration. However, the coloration did show subjective improvement in the contrast among tissues. The results showed that pigment addition prior to acetone dehydration, or prior to silicone impregnation, resulted in the best coloration, and that the use of Rit dye was most effective. Use of this approach was subsequently validated using human arm tissue, which consists of more tissue variety than squirrel legs.
色素在松鼠腿塑化过程中不同阶段的应用
美国联合大学生物系,田纳西州杰克逊38305摘要:塑化组织因其结构完整、易于处理和储存而广受欢迎。在整个塑化过程中,标本可能会失去自然(或添加)的颜色,因此着色的一个目标是恢复自然颜色。这项研究的第二个目的是测量颜色的变化,包括客观的和主观的颜色可视化。在5个阶段的塑化过程中,在4个确定的点添加3种色素,使用松鼠腿肌肉样品进行着色研究。通过测量欧几里得距离和CIE2000E值对12个实验组和一个未经处理的样本进行分析。统计分析表明,没有一种染色处理能恢复自然颜色。然而,在组织之间的对比中,颜色确实表现出主观的改善。结果表明,在丙酮脱水前添加颜料,或在硅酮浸渍前添加颜料,其着色效果最好,其中使用Rit染料效果最好。该方法的使用随后在人体手臂组织中得到验证,该组织比松鼠腿包含更多的组织种类。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Journal of Plastination
Journal of Plastination Health Professions-Medical Laboratory Technology
CiteScore
0.40
自引率
0.00%
发文量
0
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