Comparing RAS with and without biofloc: Transcriptional response of immune-related genes in Litopenaeus vannamei post-larvae

IF 0.2 4区农林科学 Q4 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Revista Colombiana De Ciencias Pecuarias Pub Date : 2020-01-31 DOI:10.17533/udea.rccp.v33n1a03

Jorge Soto Alcalá, Píndaro Álvarez Ruiz, Juan M Audelo Naranjo, Héctor M Esparza Leal, Irasema E Luis Villaseñor, J. A. Estrada-Godínez, A. Luna-González, C. Gámez-Jiménez, Genaro Diarte Plata

{"title":"Comparing RAS with and without biofloc: Transcriptional response of immune-related genes in Litopenaeus vannamei post-larvae","authors":"Jorge Soto Alcalá, Píndaro Álvarez Ruiz, Juan M Audelo Naranjo, Héctor M Esparza Leal, Irasema E Luis Villaseñor, J. A. Estrada-Godínez, A. Luna-González, C. Gámez-Jiménez, Genaro Diarte Plata","doi":"10.17533/udea.rccp.v33n1a03","DOIUrl":null,"url":null,"abstract":"Background: Shrimp farming is evolving from semi-intensive to hyper-intensive systems with biofloc technology and water recirculation systems. Objective: To evaluate the transcriptional response promoted by biofloc on shrimp ( Litopenaeus vannamei ) under a recirculating aquaculture system (RAS). Methods: Quantitative real-time RT-PCR was used to monitor seven key genes related to the immune system in shrimp post-larvae, reared in a RAS with and without biofloc (BF and noBF). In addition, we present for the first time nucleotide sequences of ADP-ribosylation factor 4 (LvArf4) from Litopenaeus vannamei . Results: Transcripts for penaeidin3 (Pen3), penaeidin4 (Pen4), crustin, and Toll receptor (LvToll) genes were upregulated between 3 and 24 h in both systems, and tumor necrosis factor receptor-associated factor 6 (TRAF6) in no-BF as an early response. Regarding differential expression between treatments, 13 occurrences were encountered. Nine that were higher in BF than in no-BF and four higher in no-BF than in BF. In some sample times, expression of Pen3, crustin, LvToll, TRAF6, IMD, and LvArf4 was higher in BF than in no-BF and in others, expression of Pen3, Pen4, and TRAF6 was higher in no-BF than in BF. Conclusions: BF modulates the transcription of genes related to the immune response in shrimp as an early response. However, the RAS with no-BF promotes a similar response. Keywords: antimicrobial peptide; aquaculture; biofloc; gene expression; Penaeidin; RAS; shrimp; shrimp immune system. Resumen Antecedentes: Los cultivos de camaron estan evolucionando de sistemas semi-intensivos a hiper-intensivos con biofloc y con recirculacion. Objetivo: Evaluar la respuesta transcripcional promovida por el biofloc en un sistema acuicola con recirculacion (SAR). Metodos: Monitoreamos mediante RT-PCR cuantitativo siete genes relacionados con el sistema inmune en postlarvas de camaron cultivadas en un SAR con y sin biofloc (BF y no-BF). Ademas, presentamos por primera vez la secuencia de nucleotidos del factor de ribosilacion 4 de ADP (LvArf4) de Litopenaeus vannamei . Resultados: Los genes penaeidina3 (Pen3), penaeidina4 (Pen4), Crustina y Toll (LvToll) se sobre-expresaron entre las 3 y 24 h en ambos sistemas, y el factor 6 asociado al factor de necrosis tumoral (TRAF6) en BF como una respuesta temprana. Con respecto a la expresion diferencial entre los tratamientos, se presentaron 13 ocurrencias. Nueve donde el BF fue mayor que sin-BF y cuatro donde el no-BF fue mayor que el BF. La expresion fue mas alta en BF que en no-BF en Pen3, Crustin, LvToll, TRAF6, IMD y LvArf4. En contraste, la expresion fue mayor en no-BF en Pen3, Pen4 y TRAF6. Conclusiones: el BF modula la transcripcion de los genes relacionados con la respuesta inmune en camaron como una respuesta temprana. Sin embargo, el SAR sin-BF promueve una respuesta similar. Palabras clave: aquicultura; biofloc; camaron; expresion genetica; Penaeidina; peptido antimicrobiano; SAR; sistema inmune de camaron. Resumo Antecedentes: A criacao de camaroes esta evoluindo de sistemas semi-intensivos para hiper-intensivos como tecnologia de bioflocos e sistemas de recirculacao. Objetivo: Avaliar a resposta transcricional promovida pelo biofloco em um sistema de aquicultura recirculante (SAR). Metodos: Utilizamos RT-PCR quantitativo em tempo real para monitorar sete genes-chave relacionados ao sistema imune em pos-larvas de camarao, criados em SAR com e sem bioflocos (BF e no-BF). Alem disso, apresentamos pela primeira vez sequencias nucleotidicas do fator de ribosilacao do ADP 4 (LvArf4) de Litopenaeus vannamei . Resultados: Os resultados mostraram que o Penaeidina3 (PEN3), Penaeidina4 (Pen4), Crustina e Toll genes (LvToll) foram sobre-expressos entre 3 e 24 h em ambos os sistemas, e o Factor de Necrose do Receptor 6 associado e protuberância (TRAF6) no BF como uma resposta precoce. Com relacao a expressao diferencial entre tratamentos, 13 ocorrencias foram apresentadas. Nove onde o BF foi maior do que os nao-BF e quatro onde o nao-BF foi maior do que o BF. A expressao foi maior do que em BF nao-BF em Pen3, Crustin, LvToll, TRAF6, IMD e LvArf4. Em contraste, a expressao foi mais elevada no nao-BF em Pen3, Pen4 e TRAF6. Conclusoes: O BF modula a transcricao de resposta imune relacionada no camarao como um genes de resposta precoce. No entanto, o SAR nao BF promove uma resposta semelhante. Palavras-chave: aquicultura; biofloco; camarao; expressao genetica; Penaeidina; peptideo antimicrobiano; SAR; sistema imunologico do camarao.","PeriodicalId":49613,"journal":{"name":"Revista Colombiana De Ciencias Pecuarias","volume":"33 1","pages":"32-43"},"PeriodicalIF":0.2000,"publicationDate":"2020-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Revista Colombiana De Ciencias Pecuarias","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.17533/udea.rccp.v33n1a03","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"AGRICULTURE, DAIRY & ANIMAL SCIENCE","Score":null,"Total":0}

引用次数: 1

Abstract

Background: Shrimp farming is evolving from semi-intensive to hyper-intensive systems with biofloc technology and water recirculation systems. Objective: To evaluate the transcriptional response promoted by biofloc on shrimp ( Litopenaeus vannamei ) under a recirculating aquaculture system (RAS). Methods: Quantitative real-time RT-PCR was used to monitor seven key genes related to the immune system in shrimp post-larvae, reared in a RAS with and without biofloc (BF and noBF). In addition, we present for the first time nucleotide sequences of ADP-ribosylation factor 4 (LvArf4) from Litopenaeus vannamei . Results: Transcripts for penaeidin3 (Pen3), penaeidin4 (Pen4), crustin, and Toll receptor (LvToll) genes were upregulated between 3 and 24 h in both systems, and tumor necrosis factor receptor-associated factor 6 (TRAF6) in no-BF as an early response. Regarding differential expression between treatments, 13 occurrences were encountered. Nine that were higher in BF than in no-BF and four higher in no-BF than in BF. In some sample times, expression of Pen3, crustin, LvToll, TRAF6, IMD, and LvArf4 was higher in BF than in no-BF and in others, expression of Pen3, Pen4, and TRAF6 was higher in no-BF than in BF. Conclusions: BF modulates the transcription of genes related to the immune response in shrimp as an early response. However, the RAS with no-BF promotes a similar response. Keywords: antimicrobial peptide; aquaculture; biofloc; gene expression; Penaeidin; RAS; shrimp; shrimp immune system. Resumen Antecedentes: Los cultivos de camaron estan evolucionando de sistemas semi-intensivos a hiper-intensivos con biofloc y con recirculacion. Objetivo: Evaluar la respuesta transcripcional promovida por el biofloc en un sistema acuicola con recirculacion (SAR). Metodos: Monitoreamos mediante RT-PCR cuantitativo siete genes relacionados con el sistema inmune en postlarvas de camaron cultivadas en un SAR con y sin biofloc (BF y no-BF). Ademas, presentamos por primera vez la secuencia de nucleotidos del factor de ribosilacion 4 de ADP (LvArf4) de Litopenaeus vannamei . Resultados: Los genes penaeidina3 (Pen3), penaeidina4 (Pen4), Crustina y Toll (LvToll) se sobre-expresaron entre las 3 y 24 h en ambos sistemas, y el factor 6 asociado al factor de necrosis tumoral (TRAF6) en BF como una respuesta temprana. Con respecto a la expresion diferencial entre los tratamientos, se presentaron 13 ocurrencias. Nueve donde el BF fue mayor que sin-BF y cuatro donde el no-BF fue mayor que el BF. La expresion fue mas alta en BF que en no-BF en Pen3, Crustin, LvToll, TRAF6, IMD y LvArf4. En contraste, la expresion fue mayor en no-BF en Pen3, Pen4 y TRAF6. Conclusiones: el BF modula la transcripcion de los genes relacionados con la respuesta inmune en camaron como una respuesta temprana. Sin embargo, el SAR sin-BF promueve una respuesta similar. Palabras clave: aquicultura; biofloc; camaron; expresion genetica; Penaeidina; peptido antimicrobiano; SAR; sistema inmune de camaron. Resumo Antecedentes: A criacao de camaroes esta evoluindo de sistemas semi-intensivos para hiper-intensivos como tecnologia de bioflocos e sistemas de recirculacao. Objetivo: Avaliar a resposta transcricional promovida pelo biofloco em um sistema de aquicultura recirculante (SAR). Metodos: Utilizamos RT-PCR quantitativo em tempo real para monitorar sete genes-chave relacionados ao sistema imune em pos-larvas de camarao, criados em SAR com e sem bioflocos (BF e no-BF). Alem disso, apresentamos pela primeira vez sequencias nucleotidicas do fator de ribosilacao do ADP 4 (LvArf4) de Litopenaeus vannamei . Resultados: Os resultados mostraram que o Penaeidina3 (PEN3), Penaeidina4 (Pen4), Crustina e Toll genes (LvToll) foram sobre-expressos entre 3 e 24 h em ambos os sistemas, e o Factor de Necrose do Receptor 6 associado e protuberância (TRAF6) no BF como uma resposta precoce. Com relacao a expressao diferencial entre tratamentos, 13 ocorrencias foram apresentadas. Nove onde o BF foi maior do que os nao-BF e quatro onde o nao-BF foi maior do que o BF. A expressao foi maior do que em BF nao-BF em Pen3, Crustin, LvToll, TRAF6, IMD e LvArf4. Em contraste, a expressao foi mais elevada no nao-BF em Pen3, Pen4 e TRAF6. Conclusoes: O BF modula a transcricao de resposta imune relacionada no camarao como um genes de resposta precoce. No entanto, o SAR nao BF promove uma resposta semelhante. Palavras-chave: aquicultura; biofloco; camarao; expressao genetica; Penaeidina; peptideo antimicrobiano; SAR; sistema imunologico do camarao.

查看原文本刊更多论文

有和无生物块的RAS比较：凡纳滨对虾幼虫免疫相关基因的转录反应

背景:利用生物絮团技术和水循环系统，虾类养殖正在从半集约化向超集约化发展。目的:评价生物絮团对循环水养殖对虾(Litopenaeus vannamei)的转录反应。方法:采用实时荧光定量RT-PCR技术，对有无生物团块(BF和noBF)饲养的对虾幼虫进行免疫系统相关的7个关键基因的检测。此外，我们首次获得了凡纳滨对虾adp -核糖基化因子4 (LvArf4)的核苷酸序列。结果:在两种系统中，penaeidin3 (Pen3)、penaeidin4 (Pen4)、外壳蛋白和Toll受体(LvToll)基因的转录本在3 - 24小时之间上调，而在无bf中，肿瘤坏死因子受体相关因子6 (TRAF6)的转录本在早期反应中上调。不同处理间的差异表达有13例。有9个品种的BF值高于无BF值，4个品种的BF值高于无BF值。在某些样本时间内，Pen3、crustin、LvToll、TRAF6、IMD和LvArf4在BF组的表达高于无BF组，在其他样本时间内，Pen3、Pen4和TRAF6在无BF组的表达高于BF组。结论:BF可作为一种早期应答调节对虾免疫应答相关基因的转录。然而，没有bf的RAS促进了类似的反应。关键词:抗菌肽;水产养殖;biofloc;基因表达;Penaeidin;RAS;虾;虾的免疫系统。前情提要:三种植物的进化和系统的半集约化和高集约化生物循环。目的:探讨针刺系统再循环(SAR)中不同生物群落的转录促进作用。方法:采用RT-PCR技术对大鼠体外培养后免疫系统相关基因进行定量分析，并与生物群落(BF / no-BF)进行比较。admas，呈报了pprimeras la secuencia de nucleotidos del factor de ribosilacion 4 de ADP (LvArf4) de Litopenaeus vannamei。结果:Los基因penaeidina3 (Pen3)、penidina4 (Pen4)、Crustina y Toll (LvToll)在两种肿瘤组织中均在3 ~ 24 h呈阳性表达，而el - 6相关肿瘤坏死因子(TRAF6)在两种肿瘤组织中均呈阳性表达。相对于不同的表达方式，我们有13种不同的货币。Nueve donde el BF - mayor que sinbf - cuatro donde el no-BF - mayor que el BF。在Pen3, Crustin, LvToll, TRAF6, IMD和LvArf4中，La表达量在BF中是不存在的。相反，表达式fue mayor En no-BF En Pen3, Pen4 y TRAF6。结论:el - BF调控与慢性呼吸道感染相关基因转录有关。在禁运期间，中国的SAR - bf促销活动也有类似的情况。鱼:水产养殖;biofloc;对虾;表达式的值遗传;Penaeidina;peptido antimicrobiano;特别行政区;卡马龙免疫系统。简历前言:一个关键的camaros的进化，系统，半密集，准密集的como技术，生物技术，系统，循环。目的:研究海参在循环水养殖系统(SAR)中的应用。方法:利用RT-PCR技术定量检测与系统免疫系统相关的部分基因，如幼虫后免疫系统、免疫系统、免疫系统相关基因、免疫系统相关基因(BF和no-BF)。Alem disso，报告了南美对虾adp4 (LvArf4)的初始核苷酸序列。结果:结果表明，在本组患者中，penaeidin3 (PEN3)、penaeidin4 (Pen4)、Crustina e Toll基因(LvToll)表达量最多，3、24例患者中，坏死因子受体6相关因子(TRAF6)表达量最多。Com的相关性和表达不同的中心处理，13种不同的报告。半半半半半半半半半半半半半半半半半半半半半半半半半半半半半半半。一种主要表达因子为BF - nbf - nbf - mpen3, cristin, LvToll, TRAF6, IMD和LvArf4。相比之下，主要的高通量数据为:1、3、4、TRAF6。结论:O - BF模组与反应性早孕的免疫相关，而与反应性早孕的基因无关。不进入，不进入，不进入，不进入，不进入，不进入。Palavras-chave: aquicultura;biofloco;camarao;expressao遗传;Penaeidina;peptideo antimicrobiano;特别行政区;camarao免疫学系统。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Revista Colombiana De Ciencias Pecuarias AGRICULTURE, DAIRY & ANIMAL SCIENCE-

CiteScore

0.80

自引率

0.00%

发文量

审稿时长

6-12 weeks

期刊介绍： The editors of Revista Colombiana de Ciencias Pecuarias (RCCP) welcome the submission of original manuscripts on experimental and clinical studies associated with the broad areas of animal sciences and veterinary medicine as they interface with biochemistry, molecular biology, physiology, pharmacology, toxicology, pathology, microbiology, parasitology, immunology and epidemiology. The scope of the journal includes studies of basic and applied research in animal management and production, feeding and nutrition, reproduction, breeding, genetics, animal welfare and behavior; as well as animal production focussed from biotechnology, soil science, agrostology, silvopastoral systems, livestock economics and the environment. The criteria for acceptance of papers submitted for publication are originality, quality and clarity of the content. Each contribution must be based on original, unpublished research that has not been simultaneously submitted to other journals. All papers will be peer reviewed. All authors bear responsibility for ensuring the integrity and quality of their reported research. It is the author''s responsibility to secure permission to use figures or tables that have been published elsewhere. Contributions may be classified as original research, review, rapid communication, clinical case studies or methodological articles, as well as news/commentaries or letters to the editor. Most review articles are invited by the editor. Authors interested in submitting a review article should contact the corresponding editor. Rapid publication of original manuscripts is a goal of the journal. Manuscripts must be written in English. Each manuscript is considered for publication with the understanding that it has not been simultaneously submitted to any other journal. Upon acceptance for publication, papers are subject to editorial review and revision.