Optical tweezers — from calibration to applications: a tutorial

IF 25.2 1区物理与天体物理 Q1 OPTICS

Advances in Optics and Photonics Pub Date : 2020-04-10 DOI:10.1364/aop.394888

J. Gieseler, Juan Ruben Gomez-Solano, A. Magazzù, I. Pérez Castillo, Laura Pérez García, M. Gironella-Torrent, X. Viader-Godoy, F. Ritort, G. Pesce, A. Arzola, K. Volke-Sepúlveda, G. Volpe

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引用次数: 82

Abstract

Since their invention in 1986 by Arthur Ashkin and colleagues, optical tweezers have become an essential tool in several fields of physics, spectroscopy, biology, nanotechnology, and thermodynamics. In this Tutorial, we provide a primer on how to calibrate optical tweezers and how to use them for advanced applications. After a brief general introduction on optical tweezers, we focus on describing and comparing the various available calibration techniques. Then, we discuss some cutting-edge applications of optical tweezers in a liquid medium, namely to study single-molecule and single-cell mechanics, microrheology, colloidal interactions, statistical physics, and transport phenomena. Finally, we consider optical tweezers in vacuum, where the absence of a viscous medium offers vastly different dynamics and presents new challenges. We conclude with some perspectives for the field and the future application of optical tweezers. This Tutorial provides both a step-by-step guide ideal for non-specialists entering the field and a comprehensive manual of advanced techniques useful for expert practitioners. All the examples are complemented by the sample data and software necessary to reproduce them.

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光学镊子-从校准到应用:教程

自1986年亚瑟·阿什金及其同事发明光镊以来，光镊已成为物理学、光谱学、生物学、纳米技术和热力学等多个领域的重要工具。在本教程中，我们提供了如何校准光学镊子以及如何将其用于高级应用的入门。在简要介绍光镊之后，我们将重点描述和比较各种可用的校准技术。然后，我们讨论了光镊在液体介质中的一些前沿应用，即研究单分子和单细胞力学、微流变学、胶体相互作用、统计物理和输运现象。最后，我们考虑真空中的光镊，其中没有粘性介质提供了截然不同的动力学并提出了新的挑战。最后，对光学镊子的应用前景进行了展望。本教程为进入该领域的非专业人员提供了理想的分步指南，并为专家从业者提供了有用的先进技术的全面手册。所有的例子都补充了样本数据和必要的软件来复制它们。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Advances in Optics and Photonics OPTICS-

CiteScore

56.60

自引率

0.00%

发文量

期刊介绍： Advances in Optics and Photonics (AOP) is an all-electronic journal that publishes comprehensive review articles and multimedia tutorials. It is suitable for students, researchers, faculty, business professionals, and engineers interested in optics and photonics. The content of the journal covers advancements in these fields, ranging from fundamental science to engineering applications. The journal aims to capture the most significant developments in optics and photonics. It achieves this through long review articles and comprehensive tutorials written by prominent and respected authors who are at the forefront of their fields. The journal goes beyond traditional text-based articles by enhancing the content with multimedia elements, such as animation and video. This multimedia approach helps to enhance the understanding and visualization of complex concepts. AOP offers dedicated article preparation and peer-review support to assist authors throughout the publication process. This support ensures that the articles meet the journal's standards and are well-received by readers. Additionally, AOP welcomes comments on published review articles, encouraging further discussions and insights from the scientific community. In summary, Advances in Optics and Photonics is a comprehensive journal that provides authoritative and accessible content on advancements in optics and photonics. With its diverse range of articles, multimedia enhancements, and dedicated support, AOP serves as a valuable resource for professionals and researchers in these fields.