Synthetic biology-based optogenetic approaches to control therapeutic designer cells

IF 3.4 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Current Opinion in Systems Biology Pub Date : 2021-12-01 DOI:10.1016/j.coisb.2021.100396

Maysam Mansouri , Martin Fussenegger

引用次数: 5

Abstract

Optogenetics uses light as a traceless inducer to remotely control cellular behavior with high safety and spatiotemporal precision, and its implementation for therapeutic synthetic biology enable customizable user-defined remedial outputs to be generated from suitably engineered cells. Here, we focus on non-neural optogenetics, describing the tools and strategies available to engineer light-responsive, therapeutic mammalian designer cells and highlighting recent advances in design and translational applications, including cell and gene therapies. We also discuss current limitations in engineering genetically encoded light-sensitive systems and suggest some possible solutions.

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基于合成生物学的光遗传学方法控制治疗设计细胞

光遗传学利用光作为一种无痕诱导剂，以高安全性和时空精度远程控制细胞行为，其在治疗性合成生物学中的实施使定制的用户定义的补救输出能够从适当的工程细胞中产生。在这里，我们专注于非神经光遗传学，描述了可用于设计光响应的治疗性哺乳动物设计细胞的工具和策略，并强调了设计和转化应用的最新进展，包括细胞和基因治疗。我们还讨论了目前工程遗传编码光敏系统的局限性，并提出了一些可能的解决方案。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Current Opinion in Systems Biology Mathematics-Applied Mathematics

CiteScore

7.10

自引率

2.70%

发文量

期刊介绍： Current Opinion in Systems Biology is a new systematic review journal that aims to provide specialists with a unique and educational platform to keep up-to-date with the expanding volume of information published in the field of Systems Biology. It publishes polished, concise and timely systematic reviews and opinion articles. In addition to describing recent trends, the authors are encouraged to give their subjective opinion on the topics discussed. As this is such a broad discipline, we have determined themed sections each of which is reviewed once a year. The following areas will be covered by Current Opinion in Systems Biology: -Genomics and Epigenomics -Gene Regulation -Metabolic Networks -Cancer and Systemic Diseases -Mathematical Modelling -Big Data Acquisition and Analysis -Systems Pharmacology and Physiology -Synthetic Biology -Stem Cells, Development, and Differentiation -Systems Biology of Mold Organisms -Systems Immunology and Host-Pathogen Interaction -Systems Ecology and Evolution