Inhibition of Cancer Cell Migration and Glycolysis by Terahertz Wave Modulation via Altered Chromatin Accessibility

IF 11 1区综合性期刊 Q1 Multidisciplinary

Research Pub Date : 2022-07-14 DOI:10.34133/2022/9860679

Lan Sun, Yangmei Li, Yun Yu, Peiliang Wang, Sheng-tao Zhu, K. Wu, Yan Liu, Ruixing Wang, L. Min, Cha-Wei Chang

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引用次数: 5

Abstract

Metastasis and metabolic disorders contribute to most cancer deaths and are potential drug targets in cancer treatment. However, corresponding drugs inevitably induce myeloid suppression and gastrointestinal toxicity. Here, we report a nonpharmaceutical and noninvasive electromagnetic intervention technique that exhibited long-term inhibition of cancer cells. Firstly, we revealed that optical radiation at the specific wavelength of 3.6 μm (i.e., 83 THz) significantly increased binding affinity between DNA and histone via molecular dynamics simulations, providing a theoretical possibility for THz modulation- (THM-) based cancer cell intervention. Subsequent cell functional assays demonstrated that low-power 3.6 μm THz wave could successfully inhibit cancer cell migration by 50% and reduce glycolysis by 60%. Then, mRNA sequencing and assays for transposase-accessible chromatin using sequencing (ATAC-seq) indicated that low-power THM at 3.6 μm suppressed the genes associated with glycolysis and migration by reducing the chromatin accessibility of certain gene loci. Furthermore, THM at 3.6 μm on HCT-116 cancer cells reduced the liver metastasis by 60% in a metastatic xenograft mouse model by splenic injection, successfully validated the inhibition of cancer cell migration by THM in vivo. Together, this work provides a new paradigm for electromagnetic irradiation-induced epigenetic changes and represents a theoretical basis for possible innovative therapeutic applications of THM as the future of cancer treatments.

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通过改变染色质可及性的太赫兹波调制抑制癌症细胞迁移和糖酵解

转移和代谢紊乱导致大多数癌症死亡，是癌症治疗的潜在药物靶点。然而，相应的药物不可避免地会引起骨髓抑制和胃肠道毒性。在此，我们报道了一种非药物和无创的电磁干预技术，该技术表现出对癌症细胞的长期抑制作用。首先，我们揭示了3.6特定波长的光辐射 μm（即83 THz）通过分子动力学模拟显著增加DNA和组蛋白之间的结合亲和力，为基于THz调节（THM-）的癌症细胞干预提供了理论可能性。随后的细胞功能测定表明，低功率3.6 μm THz波可成功抑制癌症细胞迁移50%，减少糖酵解60%。然后，信使核糖核酸测序和使用测序的转座酶可及染色质测定（ATAC-seq）表明，3.6 μm通过降低某些基因位点的染色质可及性来抑制与糖酵解和迁移相关的基因。此外，THM为3.6 μm对HCT-116癌症细胞的抑制作用，使脾注射转移性异种移植小鼠模型的肝转移减少60%，成功验证了THM对癌症细胞迁移的抑制作用。总之，这项工作为电磁辐射诱导的表观遗传学变化提供了一种新的范式，并为THM作为癌症治疗的未来可能的创新治疗应用提供了理论基础。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Research Multidisciplinary-Multidisciplinary

CiteScore

13.40

自引率

3.60%

发文量

审稿时长

14 weeks

期刊介绍： Research serves as a global platform for academic exchange, collaboration, and technological advancements. This journal welcomes high-quality research contributions from any domain, with open arms to authors from around the globe. Comprising fundamental research in the life and physical sciences, Research also highlights significant findings and issues in engineering and applied science. The journal proudly features original research articles, reviews, perspectives, and editorials, fostering a diverse and dynamic scholarly environment.