The Effects of Heat Shock Proteins on Delivery of HIV-1 Nef Antigen in Mammalian Cells

Abstract

. After 48 hours, their transfection efficiency was evaluated using fluorescent microscopy and flow cytometry. Results: The pEGFP-nef , - hsp27, -hsp27-nef, -hsp70 and - hsp70 - nef constructs were successfully prepared in large scale and high purity. The results of cell transfection with each construct showed that the percentages of Nef-GFP, Hsp27-GFP, Hsp27-Nef-GFP, Hsp70-GFP, Hsp70-Nef-GFP, Hsp70-GFP + Nef-GFP and GFP expression were 53.1  0.2, 64.22  0.8, 57.1  0.7, 68.8  1.0, 61.7  0.7, 77.4  1.5 and 81.8  1.8, respectively. Conclusion: These data showed that the potency of Hsp70 was more than Hsp27 for increasing Nef-GFP expression in HEK-293T cells. Moreover, the delivery of pEGFP-nef along with pEGFP-hsp70 increased the rate of GFP population in the cells as compared to that in pEGFP-hsp70-nef. These constructs will be used for development of HIV-1 DNA vaccine in animal model in near Future.