Spatially guided endothelial tubulogenesis by laser-induced side transfer (LIST) bioprinting of HUVECs

Q1 Computer Science

Bioprinting Pub Date : 2022-12-01 DOI:10.1016/j.bprint.2022.e00240

Hamid Ebrahimi Orimi , Erika Hooker , Sivakumar Narayanswamy , Bruno Larrivée , Christos Boutopoulos

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引用次数: 1

Abstract

The ability to bioprint microvasculature networks is central for drug screening and for tissue engineering applications. Here we used a newly developed bioprinting technology, termed laser-induced side transfer (LIST), to print human umbilical vein endothelial cells (HUVECs) and to spatially guide endothelial tubulogenesis. We investigated the effect of three bioprinting matrices (fibrin, Matrigel and Matrigel/thrombin) on HUVECs self-assembly. Furthermore, we studied the effect of pro- and anti-angiogenic compounds on sprouting angiogenesis and tubulogenesis. We found that HUVECs self-assembly is optimal on Matrigel/thrombin due to the formation of fibrin stripes that enhance HUVECs confinement and adhesion. Importantly, we showed that treatment of printed HUVEC lines with the anti-angiogenic factor bone morphogenetic protein 9 (BMP9) significantly improves the percentage of lumen coverage. Our results showcase LIST as a powerful bioprinting technology to study tubulogenesis and to screen compounds targeting microvasculature pathologies.

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激光诱导侧转移(LIST)生物打印HUVECs的空间引导内皮小管形成

生物打印微血管网络的能力是药物筛选和组织工程应用的核心。在这里，我们使用了一种新开发的生物打印技术，称为激光诱导侧转移(LIST)，来打印人脐静脉内皮细胞(HUVECs)，并在空间上引导内皮小管的形成。我们研究了三种生物打印基质(纤维蛋白、Matrigel和Matrigel/凝血酶)对HUVECs自组装的影响。此外，我们还研究了促血管生成和抗血管生成化合物对发芽血管生成和小管生成的影响。我们发现，由于纤维蛋白条纹的形成增强了HUVECs的约束和粘附性，HUVECs在Matrigel/凝血酶上的自组装是最佳的。重要的是，我们发现用抗血管生成因子骨形态发生蛋白9 (BMP9)处理打印的HUVEC细胞系可显著提高管腔覆盖率。我们的研究结果表明，LIST是一种强大的生物打印技术，可用于研究微管发生和筛选针对微血管病变的化合物。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Bioprinting Computer Science-Computer Science Applications

CiteScore

11.50

自引率

0.00%

发文量

审稿时长

68 days

期刊介绍： Bioprinting is a broad-spectrum, multidisciplinary journal that covers all aspects of 3D fabrication technology involving biological tissues, organs and cells for medical and biotechnology applications. Topics covered include nanomaterials, biomaterials, scaffolds, 3D printing technology, imaging and CAD/CAM software and hardware, post-printing bioreactor maturation, cell and biological factor patterning, biofabrication, tissue engineering and other applications of 3D bioprinting technology. Bioprinting publishes research reports describing novel results with high clinical significance in all areas of 3D bioprinting research. Bioprinting issues contain a wide variety of review and analysis articles covering topics relevant to 3D bioprinting ranging from basic biological, material and technical advances to pre-clinical and clinical applications of 3D bioprinting.