Interaction between Vascular Endothelial Growth Factor-A (rs2010963) Gene Polymorphisms and Dietary Diversity Score on Cardiovascular Risk Factors in Patients with Metabolic Syndrome

IF 2 4区 医学 Q3 GENETICS & HEREDITY
M. Abbasalizad Farhangi, M. Vajdi, L. Nikniaz, Zeinab Nikniaz
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引用次数: 12

Abstract

Background: The vascular endothelial growth factor-A (VEGFA) family of cytokines regulates proliferation, angiogenesis, and migration of endothelial cells, increases vascular permeability, and controls thrombogenicity. Recent studies have suggested that the VEGFA gene plays an important role in the pathogenesis of metabolic syndrome and its related disorders. Dietary diversity score (DDS) has also been shown to have potential favorable effects against features of metabolic syndrome. This study examined the interactions between +405 VEGFA C/G (rs2010963) polymorphism and DDS on the metabolic and biochemical profile of metabolic syndrome. Therefore, in the current study, we aimed to evaluate the interaction between DDS and VEGFA rs2010963 gene polymorphisms in modification of metabolic risk factors including serum lipids, blood pressure, serum adiponectin, and matrix metalloproteinase (MMP)-3 concentrations in patients with metabolic syndrome. Methods and Materials: In the current cross-sectional study, 254 patients with metabolic syndrome were recruited. Measurements of blood pressure, anthropometric parameters, and dietary intakes were performed and the DDS was calculated. Biochemical variables including serum adiponectin concentrations, lipid profile, serum glucose, and MMP-3 concentrations were measured by enzyme-linked immunosorbent assay method (ELISA) and enzymatic colorimetric methods. Determination of +405 C/G VEGFA gene polymorphisms was performed using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique. Results: Patients in the lowest DDS quartile had higher insulin and homeostatic model assessment of insulin resistance (HOMA-IR), while patients in the highest DDS quartile had higher quantitative insulin check index (QUICKI; p < 0.05). Higher serum triglyceride and systolic blood pressure (SBP) values and lower serum adiponectin concentrations were also observed in lower DDS quartiles (p < 0.05). Patients with the CC genotype in the VEGFA rs2010963 polymorphism had significantly higher body mass index (BMI), fasting blood glucose, aspartate aminotransferase (AST), and alanine aminotransferase (ALT; p < 0.05) compared to patients with the other 2 genotypes. In lower quartiles of DDS, 30% of patients with metabolic syndrome had the GG genotype, while 30.4 and 30.8% of patients with metabolic syndrome in higher DDS quartiles had GC and CC genotypes, respectively (p = 0.04). Conclusion: Our study found lower insulin resistance, serum triglyceride, and SBP and higher adiponectin concentrations among patients with metabolic syndrome in highest quartiles of DDS. Moreover, patients with the CC genotype were more likely to have higher BMI, fasting blood glucose, AST, and ALT. This significant interaction gives a possible evidence of a VEGFA-DDS association that may be relevant to metabolic syndrome. Further studies are warranted to clarify the underlying mechanisms of these interactions.
血管内皮生长因子- a (rs2010963)基因多态性与代谢综合征患者心血管危险因素饮食多样性评分的相互作用
背景:血管内皮生长因子-A(VEGFA)家族的细胞因子调节内皮细胞的增殖、血管生成和迁移,增加血管通透性,并控制血栓形成。最近的研究表明,VEGFA基因在代谢综合征及其相关疾病的发病机制中发挥着重要作用。饮食多样性评分(DDS)也被证明对代谢综合征的特征具有潜在的有利影响。本研究检测了+405 VEGFA C/G(rs2010963)多态性和DDS在代谢综合征代谢和生化特征上的相互作用。因此,在本研究中,我们旨在评估DDS和VEGFA rs2010963基因多态性在代谢综合征患者代谢危险因素(包括血脂、血压、血清脂联素和基质金属蛋白酶(MMP)-3浓度)改变中的相互作用。方法和材料:在目前的横断面研究中,254名代谢综合征患者被招募。测量血压、人体测量参数和饮食摄入量,并计算DDS。采用酶联免疫吸附测定法(ELISA)和酶比色法测定血清脂联素浓度、血脂、血糖和MMP-3浓度等生化指标。采用聚合酶链式反应-限制性片段长度多态性(PCR-RFLP)技术测定+405 C/G VEGFA基因多态性。结果:DDS最低四分位数的患者具有较高的胰岛素和胰岛素抵抗稳态模型评估(HOMA-IR),而DDS最高四分位数的患者具有较高的胰岛素定量检查指数(QUICKI;p<0.05)。DDS较低四分位数也观察到较高的血清甘油三酯和收缩压(SBP)值和较低的血清脂联素浓度(p<0.05)。VEGFA rs2010963多态性中的CC基因型患者具有显著较高的体重指数(BMI),与其他2种基因型的患者相比,空腹血糖、天冬氨酸转氨酶(AST)和丙氨酸转氨酶(ALT;p<0.05)。在DDS较低的四分位数中,30%的代谢综合征患者具有GG基因型,而在DDS较高的四分位中,分别有30.4%和30.8%的代谢综合症患者具有GC和CC基因型(p=0.04),在DDS最高四分位数的代谢综合征患者中,SBP和更高的脂联素浓度。此外,CC基因型患者更有可能具有更高的BMI、空腹血糖、AST和ALT。这种显著的相互作用提供了可能与代谢综合征相关的VEGFA-DDS相关性的可能证据。需要进一步的研究来阐明这些相互作用的潜在机制。
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来源期刊
Lifestyle Genomics
Lifestyle Genomics Agricultural and Biological Sciences-Food Science
CiteScore
4.00
自引率
7.70%
发文量
11
审稿时长
28 weeks
期刊介绍: Lifestyle Genomics aims to provide a forum for highlighting new advances in the broad area of lifestyle-gene interactions and their influence on health and disease. The journal welcomes novel contributions that investigate how genetics may influence a person’s response to lifestyle factors, such as diet and nutrition, natural health products, physical activity, and sleep, amongst others. Additionally, contributions examining how lifestyle factors influence the expression/abundance of genes, proteins and metabolites in cell and animal models as well as in humans are also of interest. The journal will publish high-quality original research papers, brief research communications, reviews outlining timely advances in the field, and brief research methods pertaining to lifestyle genomics. It will also include a unique section under the heading “Market Place” presenting articles of companies active in the area of lifestyle genomics. Research articles will undergo rigorous scientific as well as statistical/bioinformatic review to ensure excellence.
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