Comparative transcriptomic analysis of gene expression profiles in the liver and spleen of American bullfrog (Lithobates catesbeianus) in response to Citrobacter freundii infection

IF 2.3 3区 农林科学 Q2 FISHERIES
Peikui Yang, Yuzhong Zheng, Xianghui Zou, Yanjie Sun, Yaqun Liu
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引用次数: 0

Abstract

In recent years, bullfrog (Lithobates catesbeianus) has become one of the most commercially important amphibians for aquaculture in China, and its annual production has grown rapidly. However, bacterial diseases caused by Citrobacter freundii infection have resulted in enormous economic losses. The present study used RNA sequencing technology to analyze the transcriptomic profile and differentially expressed genes (DEGs) in the bullfrog liver and spleen post C. freundii infection. De novo assembly resulted in the generation of 77,556 L. catesbeianus transcripts, and 49,421 of these genes were successfully annotated to the databases. Overall, 1487 DEGs were identified in the liver group, including 788 upregulated and 709 downregulated DEGs. A total of 799 DEGs, including 311 upregulated and 488 downregulated genes, were detected in the spleen groups. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses revealed significantly enriched pathways, including pathways involved in metabolism and immune responses. The processes in which the DEGs were enriched were those related to metabolic activities, including the lipid metabolism pathway, amino acid pathway, immune-related pathway, and inflammatory responses. The results of this study provide insight into the response of bullfrogs against C. freundii at the transcriptome level and reveal the mechanism of the immune response against C. freundii.

Abstract Image

美国牛蛙肝脏和脾脏对弗氏柠檬酸杆菌感染反应基因表达谱的比较转录组学分析
近年来,牛蛙(Lithobates catesbeianus)已成为中国水产养殖中最具商业价值的两栖动物之一,年产量增长迅速。然而,由弗氏柠檬酸杆菌(Citrobacter freundii)感染引起的细菌性疾病造成了巨大的经济损失。本研究利用RNA测序技术分析了牛蛙肝脏和脾脏感染C. freundii后的转录组概况和差异表达基因(DEGs)。从头组装产生了 77,556 个 L. catesbeianus 转录本,其中 49,421 个基因被成功地注释到数据库中。肝脏组共鉴定出1487个DEGs,包括788个上调DEGs和709个下调DEGs。脾脏组共检测到 799 个 DEGs,包括 311 个上调基因和 488 个下调基因。基因本体(GO)和京都基因和基因组百科全书(KEGG)分析表明了显著富集的通路,包括参与代谢和免疫反应的通路。DEGs富集的过程与代谢活动有关,包括脂质代谢途径、氨基酸途径、免疫相关途径和炎症反应。这项研究的结果从转录组水平上揭示了牛蛙对C. freundii的反应,并揭示了牛蛙对C. freundii的免疫反应机制。
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来源期刊
CiteScore
5.90
自引率
7.10%
发文量
69
审稿时长
2 months
期刊介绍: The Journal of the World Aquaculture Society is an international scientific journal publishing original research on the culture of aquatic plants and animals including: Nutrition; Disease; Genetics and breeding; Physiology; Environmental quality; Culture systems engineering; Husbandry practices; Economics and marketing.
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