Two Sets of Compound Complex Driving for High Efficiency of Nonintegration Reprogramming of Human Fibroblasts.

IF 1.2 4区医学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Cellular reprogramming Pub Date : 2022-03-07 DOI:10.1089/cell.2021.0143

Xiangyi Lin, Cuiping Rong, Shouhai Wu

引用次数: 0

Abstract

Currently, plentiful chemical-assisted methods have been applied for mouse induced pluripotent stem cells (iPSCs). It has been reported that small-molecule compounds can only reprogram mouse embryonic fibroblasts into mouse chemically induced pluripotent stem cells (mouse CiPSCs). However, human CiPSCs have not been reported. Therefore, it is still necessary to search for safer chemically assisted human pluripotent stem cells, which might realize the potential of human iPSCs. Here, we developed two sets of chemical cocktails to greatly improve the induction efficiency of human nonintegrated iPSCs, including the 4 compound mixture (4M) and the 5 compound mixture (4MI). These two sets of complex driving strategies might greatly improve the reprogramming efficiency to generate integration-free iPSCs.

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两组复合复合物驱动高效的人成纤维细胞非整合重编程。

目前，多种化学辅助方法已应用于小鼠诱导多能干细胞(iPSCs)的培养。据报道，小分子化合物只能将小鼠胚胎成纤维细胞重编程为小鼠化学诱导多能干细胞(小鼠CiPSCs)。然而，人类CiPSCs尚未报道。因此，仍有必要寻找更安全的化学辅助人类多能干细胞，以实现人类多能干细胞的潜力。在此，我们开发了两套化学混合物，包括4化合物混合物(4M)和5化合物混合物(4MI)，大大提高了人非整合iPSCs的诱导效率。这两套复杂的驱动策略可能会大大提高重编程效率，以生成无集成的iPSCs。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Cellular reprogramming CELL & TISSUE ENGINEERING-BIOTECHNOLOGY & APPLIED MICROBIOLOGY

CiteScore

2.50

自引率

6.20%

发文量

审稿时长

3 months

期刊介绍： Cellular Reprogramming is the premier journal dedicated to providing new insights on the etiology, development, and potential treatment of various diseases through reprogramming cellular mechanisms. The Journal delivers information on cutting-edge techniques and the latest high-quality research and discoveries that are transforming biomedical research. Cellular Reprogramming coverage includes: Somatic cell nuclear transfer and reprogramming in early embryos Embryonic stem cells Nuclear transfer stem cells (stem cells derived from nuclear transfer embryos) Generation of induced pluripotent stem (iPS) cells and/or potential for cell-based therapies Epigenetics Adult stem cells and pluripotency.