The calcium-antagonist activity of the material released by olive pollen (PMR), tested on Ca2+-cytosolic of PE/CA-PJ15 cells

IF 2.2 3区环境科学与生态学 Q2 BIOLOGY

Aerobiologia Pub Date : 2022-11-07 DOI:10.1007/s10453-022-09768-1

Alberto Vitali, Alberto Marco Del Pino, Luca Regni, Eva Costanzi, Elisabetta Bravi, Ombretta Marconi, Primo Proietti, Carlo Alberto Palmerini

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引用次数: 0

Abstract

Inhalation of olive pollen (Olea europaea L.) is one of the main causes of allergy in Mediterranean countries and some areas of North America. The response to allergens consists in the production of inflammatory cytokines which is mediated by the deregulation of Ca²⁺ signals. In this study, the biological activity of the material released in olive pollen hydration (PMR) was tested on Ca²⁺ cytosolic of PE/CA-PJ15 cells (PJ-15). Ca²⁺ cytosolic was determined by fluorometric assay with the cell line PE/CA-PJ15 (PJ-15) labeled with the fluorescent probe FURA 2 AM. The material released in olive pollen hydration (PMR) was analyzed by HPLC for the determination of phenolic acids. PMR was subjected to fractionation by gel filtration, and the fractions with Ca²⁺-chelating activity were tested with SDS-PAGE and the single bands characterized by proteomic analysis. PMR showed high Ca²⁺-chelating activity and is able of blocking the increase Ca²⁺-cytosolic produced by thapsigargin (TG). PMR then restored Ca²⁺ homeostasis in PJ-15 cells deregulated by the endoplasmic reticulum Ca²⁺-ATPases inhibitor. It is therefore possible that PMR can antagonize the effects of allergens on Ca²⁺ cytosolic. The analytical characterization of the material released by the pollen highlighted in the pollen allergen Ole e 3 and in the p-coumaric acid the possible culprits of the Ca²⁺-antagonist activity of PMR. Furthermore, the sequence of Ole e 3 could provide information for the possible construction of a synthetic peptide to be used in an allergy-targeted Ca²⁺-antagonist therapy.

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橄榄花粉释放物质对PE/CA-PJ15细胞胞浆Ca2+的拮抗作用

吸入橄榄花粉（Olea europaea L.）是地中海国家和北美一些地区过敏的主要原因之一。对过敏原的反应包括炎性细胞因子的产生，这是由Ca2+信号的失调介导的。本研究在PE/CA-PJ15细胞（PJ-15）的Ca2+胞浆上测试了橄榄花粉水合（PMR）释放物质的生物活性。用荧光探针FURA2AM标记的PE/CA-PJ15（PJ-15）细胞，用荧光法测定细胞内Ca2+含量。用高效液相色谱法测定橄榄花粉水合过程中释放的物质酚酸。通过凝胶过滤对PMR进行分级，并用SDS-PAGE和蛋白质组学分析对具有Ca2+螯合活性的组分进行测试。PMR具有较高的Ca2+螯合活性，并能阻断茶毒碱（TG）引起的胞浆Ca2+的增加。PMR随后恢复PJ-15细胞中被内质网Ca2+-ATP酶抑制剂解除调节的Ca2+稳态。因此，PMR可能拮抗过敏原对Ca2+胞质的影响。花粉释放物质的分析特征突出了花粉过敏原Ole e 3和对香豆酸中PMR Ca2+拮抗剂活性的可能罪魁祸首。此外，Ole e 3的序列可以为可能构建用于过敏靶向Ca2+拮抗剂治疗的合成肽提供信息。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Aerobiologia 环境科学-环境科学

CiteScore

4.50

自引率

15.00%

发文量

审稿时长

18-36 weeks

期刊介绍： Associated with the International Association for Aerobiology, Aerobiologia is an international medium for original research and review articles in the interdisciplinary fields of aerobiology and interaction of human, plant and animal systems on the biosphere. Coverage includes bioaerosols, transport mechanisms, biometeorology, climatology, air-sea interaction, land-surface/atmosphere interaction, biological pollution, biological input to global change, microbiology, aeromycology, aeropalynology, arthropod dispersal and environmental policy. Emphasis is placed on respiratory allergology, plant pathology, pest management, biological weathering and biodeterioration, indoor air quality, air-conditioning technology, industrial aerobiology and more. Aerobiologia serves aerobiologists, and other professionals in medicine, public health, industrial and environmental hygiene, biological sciences, agriculture, atmospheric physics, botany, environmental science and cultural heritage.