Screening of anti-urease and antibacterial activities of root extract from Cylindropuntia cholla

Abstract

In this study, the root extract of Cylindropuntia cholla was analyzed as a possible source of substances with potential urease inhibitory and antibacterial activity. C. cholla cactus are abundant in the arid regions of Mexico and are traditionally used to treat kidney diseases, urinary tract infections, and in some cases, to combat the formation of kidney stones. Anti-urease action was evaluated from methanolic root extracts by initial velocity methods. We observed an IC50 of 2.04 mg/mL. Lineweaver-Burk pattern showed an uncompetitive inhibition. The antibacterial activity was evaluated over Klebsiella pneumoniae (ATCCTM 13883) and Staphylococcus aureus (ATCCTM 25923) and we only observed effects over S. aureus growth at 200 mg/mL of extract concentration.