Experimental analysis and modeling of single-cell time-course data

IF 3.4 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY
Eline Yafelé Bijman, Hans-Michael Kaltenbach, Jörg Stelling
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引用次数: 4

Abstract

Contemporary single-cell experiments produce vast amounts of data, but the interpretation of these data is far from straightforward. In particular, understanding mechanisms and sources of cell-to-cell variability, given highly complex and nonlinear cellular networks, precludes intuitive interpretation. It requires careful computational and mathematical analysis instead. Here, we discuss different types of single-cell data and computational, model-based methods currently used to analyze them. We argue that mechanistic models incorporating subpopulation or cell-specific parameters can help to identify sources of variation and to understand experimentally observed behaviors. We highlight how data types and qualities, together with the nonlinearity of single-cell dynamics, make it challenging to identify the correct underlying biological mechanisms and we outline avenues to address these challenges.

Abstract Image

单细胞时程数据的实验分析与建模
当代的单细胞实验产生了大量的数据,但对这些数据的解释远非直截了当。特别是,在高度复杂和非线性的细胞网络中,理解细胞间变异的机制和来源,排除了直观的解释。它需要仔细的计算和数学分析。在这里,我们讨论了不同类型的单细胞数据和目前用于分析它们的基于模型的计算方法。我们认为,结合亚种群或细胞特异性参数的机制模型可以帮助确定变异的来源,并理解实验观察到的行为。我们强调了数据类型和质量,以及单细胞动力学的非线性,如何使识别正确的潜在生物学机制具有挑战性,并概述了解决这些挑战的途径。
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来源期刊
Current Opinion in Systems Biology
Current Opinion in Systems Biology Mathematics-Applied Mathematics
CiteScore
7.10
自引率
2.70%
发文量
20
期刊介绍: Current Opinion in Systems Biology is a new systematic review journal that aims to provide specialists with a unique and educational platform to keep up-to-date with the expanding volume of information published in the field of Systems Biology. It publishes polished, concise and timely systematic reviews and opinion articles. In addition to describing recent trends, the authors are encouraged to give their subjective opinion on the topics discussed. As this is such a broad discipline, we have determined themed sections each of which is reviewed once a year. The following areas will be covered by Current Opinion in Systems Biology: -Genomics and Epigenomics -Gene Regulation -Metabolic Networks -Cancer and Systemic Diseases -Mathematical Modelling -Big Data Acquisition and Analysis -Systems Pharmacology and Physiology -Synthetic Biology -Stem Cells, Development, and Differentiation -Systems Biology of Mold Organisms -Systems Immunology and Host-Pathogen Interaction -Systems Ecology and Evolution
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