Optimisation of ultrasonic-assisted extraction and biological activity of total flavonoids from leaves of Murrayae exotica using response surface methodology

IF 2.2 4区 农林科学 Q2 HORTICULTURE
Yao Wen, Man Liu, Xueying Mai
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引用次数: 1

Abstract

ABSTRACT Murrayae exotica is a traditional Chinese medicine widely grown in southeast China. A Box–Behnken design of response surface methodology was employed to further optimise ultrasonic-assisted extraction conditions for M. exotica leaves total flavonoids (MELTF). The results showed that the optimised extraction conditions were an ultrasonic power of 240 W, an ultrasonic temperature of 60 °C, a solvent concentration of 76%, an ultrasonic time of 55 min and a liquid–solid ratio of 22 mL · g−1. Under these conditions, 8.59 ± 0.34 mg · g−1 was achieved as the mean experimental value of extraction yield, which amounts to 2.56 times that of ethanol leaching extraction. As compared to MELTF, MELTF after purification (MELPTF) had a higher purity of 9.96%, which was nearly nine times higher than that of MELTF (1.26%). As compared to MELTF, MELPTF had higher α-glucosidase and α-amylase inhibitory activities as well as DPPH· and ABTS·+ scavenging activities with IC50 values of 0.021, 0.094, 0.245 and 0.113 mg · mL−1, which are 1.33, 2.12, 3.17 and 1.78 times higher than those of MELTF (0.028, 0.199, 0.777 and 0.201 mg · mL−1). The study thus demonstrates the eligibility of MELPTF to be considered as a multifunctional bioactive ingredient having potential applications in anti-hyperglycaemic pharmaceutical formulation and as an antioxidant in functional foods.
利用响应面法优化超声辅助提取海参叶总黄酮的工艺及生物活性
野村是一种广泛生长在中国东南部的中药。采用响应面法的Box-Behnken设计,进一步优化超声辅助提取野荆叶总黄酮的工艺条件。结果表明,最佳提取条件为超声功率240 W,超声温度60℃,溶剂浓度76%,超声时间55 min,液固比22 mL·g−1。在此条件下,提取得率的平均实验值为8.59±0.34 mg·g−1,是乙醇浸提的2.56倍。与MELTF相比,纯化后的MELTF (MELPTF)纯度为9.96%,是MELTF(1.26%)的近9倍。与MELTF相比,MELPTF具有更高的α-葡萄糖苷酶和α-淀粉酶抑制活性以及DPPH·和ABTS·+清除活性,IC50值分别为0.021、0.094、0.245和0.113 mg·mL−1,分别是MELTF(0.028、0.199、0.777和0.201 mg·mL−1)的1.33、2.12、3.17和1.78倍。因此,该研究证明了MELPTF作为一种多功能生物活性成分的资格,在抗高血糖药物配方和功能食品中的抗氧化剂中具有潜在的应用前景。
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来源期刊
Folia Horticulturae
Folia Horticulturae Agricultural and Biological Sciences-Horticulture
CiteScore
3.40
自引率
0.00%
发文量
13
审稿时长
16 weeks
期刊介绍: Folia Horticulturae is an international, scientific journal published in English. It covers a broad research spectrum of aspects related to horticultural science that are of interest to a wide scientific community and have an impact on progress in both basic and applied research carried out with the use of horticultural crops and their products. The journal’s aim is to disseminate recent findings and serve as a forum for presenting views as well as for discussing important problems and prospects of modern horticulture, particularly in relation to sustainable production of high yield and quality of horticultural products, including their impact on human health.
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