Assessment of genetic diversity and population structure of the genus Vicia (Vicia L.) using simple sequence repeat markers

IF 1.1 4区农林科学 Q3 AGRICULTURE, MULTIDISCIPLINARY

Grassland Science Pub Date : 2022-03-01 DOI:10.1111/grs.12356

Wei-Hong Sun, Fei-Fei Wu, Lili Cong, Mei-Yan Jin, Xian-Guo Wang

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引用次数: 3

Abstract

The genetic diversity of the Vicia (Vicia L.) genus was analyzed using simple sequence repeat (SSR) markers. A total of 201 sampled individuals from 12 Vicia were assayed to study the genetic diversity and polymorphisms using 12 simple sequence repeat markers; 115 alleles were identified. The average observed heterozygosity (Ho) and the average expected heterozygosity (He) were 0.4283 and 0.6941, respectively. The polymorphic information content (PIC) varied from 0.8739 (V81) to 0.9579 (V97), with a mean of 0.9033, indicating that the markers were highly informative. Moreover, cluster and principal coordinate analysis (PCoA) divided the 12 species into three main clusters. Structure analysis and PCoA of Vicia narbonensis and Vicia tibetica agreed well with the cluster analysis results, and analysis of molecular variance (AMOVA) results revealed that 89% genetic variation was observed within the population and 11% was among the population. These results clarify the genetic diversity and population structure of the Vicia, providing useful information for understanding genetic variability and establishing a foundation for future breeding programs and genetic improvement.

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利用简单序列重复标记评价薇迪亚属(Vicia L.)遗传多样性和群体结构

利用SSR (simple sequence repeat)标记分析了紫杉属(Vicia L.)的遗传多样性。利用12个简单序列重复标记，对12个紫杉属201个样本的遗传多样性和多态性进行了分析;共鉴定出115个等位基因。平均观察杂合度(Ho)和平均期望杂合度(He)分别为0.4283和0.6941。多态性信息含量(PIC)在0.8739 (V81) ~ 0.9579 (V97)之间变化，平均为0.9033，表明该标记具有较高的信息性。聚类和主坐标分析(PCoA)将12种植物划分为3个主要聚类。结构分析和PCoA与聚类分析结果吻合较好，分子方差分析(AMOVA)结果表明，种群内遗传变异89%，种群间遗传变异11%。这些结果阐明了紫堇的遗传多样性和群体结构，为了解紫堇的遗传变异提供了有用的信息，并为今后的育种计划和遗传改良奠定了基础。

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来源期刊

Grassland Science Agricultural and Biological Sciences-Agronomy and Crop Science

CiteScore

2.70

自引率

7.70%

发文量

审稿时长

>12 weeks

期刊介绍： Grassland Science is the official English language journal of the Japanese Society of Grassland Science. It publishes original research papers, review articles and short reports in all aspects of grassland science, with an aim of presenting and sharing knowledge, ideas and philosophies on better management and use of grasslands, forage crops and turf plants for both agricultural and non-agricultural purposes across the world. Contributions from anyone, non-members as well as members, are welcome in any of the following fields: grassland environment, landscape, ecology and systems analysis; pasture and lawn establishment, management and cultivation; grassland utilization, animal management, behavior, nutrition and production; forage conservation, processing, storage, utilization and nutritive value; physiology, morphology, pathology and entomology of plants; breeding and genetics; physicochemical property of soil, soil animals and microorganisms and plant nutrition; economics in grassland systems.