Ji-Seob Choi, Dong-Hwi Ham, Jung-Hyun Kim, Helem Betsua Flores Marcial, Pyeong-Ho Jeong, Jin-Ho Choi, Woo-Tae Park
{"title":"Quantitative image analysis of thrombus formation in microfluidic in-vitro models","authors":"Ji-Seob Choi, Dong-Hwi Ham, Jung-Hyun Kim, Helem Betsua Flores Marcial, Pyeong-Ho Jeong, Jin-Ho Choi, Woo-Tae Park","doi":"10.1186/s40486-022-00166-3","DOIUrl":null,"url":null,"abstract":"<div><p>In this study, we present a method to quantitatively analyze the thrombus formation process through image analysis in an in vitro thrombus model with a circular cross section. The thrombus model used was designed based on the mechanism between the physical principle of wall shear rate (WSR) and thrombus formation. Image analysis was used to help visualize the thrombus formation process and calculate the thrombus area. Through this method, the thrombus formation and growth from the channel wall was demonstrated without the use of fluorescence. In addition, by dividing the image into sub-sections, the accuracy of the thrombus growth pattern was improved. The departing blood clots which are called embolus, were observed being separated from the thrombus.</p></div>","PeriodicalId":704,"journal":{"name":"Micro and Nano Systems Letters","volume":null,"pages":null},"PeriodicalIF":4.7000,"publicationDate":"2022-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://mnsl-journal.springeropen.com/counter/pdf/10.1186/s40486-022-00166-3","citationCount":"2","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Micro and Nano Systems Letters","FirstCategoryId":"1085","ListUrlMain":"https://link.springer.com/article/10.1186/s40486-022-00166-3","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"NANOSCIENCE & NANOTECHNOLOGY","Score":null,"Total":0}
引用次数: 2
Abstract
In this study, we present a method to quantitatively analyze the thrombus formation process through image analysis in an in vitro thrombus model with a circular cross section. The thrombus model used was designed based on the mechanism between the physical principle of wall shear rate (WSR) and thrombus formation. Image analysis was used to help visualize the thrombus formation process and calculate the thrombus area. Through this method, the thrombus formation and growth from the channel wall was demonstrated without the use of fluorescence. In addition, by dividing the image into sub-sections, the accuracy of the thrombus growth pattern was improved. The departing blood clots which are called embolus, were observed being separated from the thrombus.
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