Cissus quadrangularis L extract-loaded tricalcium phosphate reinforced natural polymer composite for guided bone regeneration

IF 4.2 3区医学 Q2 ENGINEERING, BIOMEDICAL

Journal of Materials Science: Materials in Medicine Pub Date : 2023-07-19 DOI:10.1007/s10856-023-06739-x

Lele Liao, Weihong Zhu, Cheng Tao, Ding Li, Minzhi Mao

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引用次数: 1

Abstract

Natural medicines plants are significant considerable attention as potential therapeutic agents for bone tissue engineering. Cissus quadrangularis L (CQ). is a potent therapeutic plant known for its own osteogenic properties. In this research work, a phytoconstituents-filled composite was produced by incorporating CQ extract with gelatin (Gel) and pectin (Pec) polymers collective through β- tricalcium phosphate (β-TCP) bioceramic via a green template method. The effect of CQ-filled composite morphology and chemical structural properties, in vitro cytotoxicity, cell proliferation, and differentiation was investigated. FTIR spectroscopic results indicated the prepared materials’ structural confirmation. The CQ extract was the alcoholic -OH merge with the hydroxyl and -NH groups in the range of 3000 cm⁻¹ to 3500 cm⁻¹. Scanning electron microscopy images showed that the β-TCP ceramic was perfectly embedded in Gel-Pec polymeric matrix, which is important for bone regeneration. In vitro cell culture results indicated that β-TCP/Gel-Pec/CQ composite provided 92.0% of a favorable substrate for mesenchymal stem cell viability. The gene expression and RT-PCR studies represent the materials with good osteogenic expression, especially the β-TCP/Gel-Pec/CQ composite is observed at 168.0% and 188.0% for RUNx2 and OCN, respectively. The result of the physicochemical characterizations and cell viability studies suggest that CQ-loaded β-TCP/Gel-Pec composite can serve as a potential biomaterial for bone tissue repair and regeneration.

Graphical Abstract

Abstract Image

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载磷酸三钙增强天然聚合物复合材料引导骨再生的研究

天然药物植物作为潜在的骨组织工程治疗剂受到广泛关注。四棱草(CQ)。是一种有效的治疗植物，以其自身的成骨特性而闻名。本研究采用绿色模板法，将CQ提取物与明胶(Gel)和果胶(Pec)聚合物通过β-磷酸三钙(β- tcp)生物陶瓷聚合，制备了一种植物成分填充复合材料。研究了cq填充复合材料的形态和化学结构特性、体外细胞毒性、细胞增殖和分化的影响。FTIR光谱结果证实了所制备材料的结构。CQ萃取物为乙醇-OH与羟基和-NH基团在3000 cm−1 ~ 3500 cm−1范围内合并。扫描电镜结果显示，β-TCP陶瓷在凝胶- pec聚合物基质中嵌入良好，对骨再生具有重要意义。体外细胞培养结果表明，β-TCP/Gel-Pec/CQ复合物为间充质干细胞提供了92.0%的有利底物。基因表达和RT-PCR研究表明，材料具有良好的成骨表达，特别是β-TCP/Gel-Pec/CQ复合物在RUNx2和OCN中分别达到168.0%和188.0%。理化表征和细胞活力研究结果表明，cq负载β-TCP/Gel-Pec复合材料可作为骨组织修复和再生的潜在生物材料。图形抽象

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来源期刊

Journal of Materials Science: Materials in Medicine 工程技术-材料科学：生物材料

CiteScore

8.00

自引率

0.00%

发文量

审稿时长

3.5 months

期刊介绍： The Journal of Materials Science: Materials in Medicine publishes refereed papers providing significant progress in the application of biomaterials and tissue engineering constructs as medical or dental implants, prostheses and devices. Coverage spans a wide range of topics from basic science to clinical applications, around the theme of materials in medicine and dentistry. The central element is the development of synthetic and natural materials used in orthopaedic, maxillofacial, cardiovascular, neurological, ophthalmic and dental applications. Special biomedical topics include biomaterial synthesis and characterisation, biocompatibility studies, nanomedicine, tissue engineering constructs and cell substrates, regenerative medicine, computer modelling and other advanced experimental methodologies.