Genetic diversity and structure analysis of Croatian garlic collection assessed by SSR markers

IF 2.2 4区农林科学 Q2 HORTICULTURE

Folia Horticulturae Pub Date : 2021-05-13 DOI:10.2478/fhort-2021-0011

D. Poljuha, M. Franić, Ines Kralj, T. Weber, Z. Šatović, D. Ban, N. Toth, G. Dumičić, S. Kereša, Camila Pinto da Cunha, S. G. Goreta Ban

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引用次数: 4

Abstract

Abstract This study examines genetic diversity and structure of a Croatian garlic germplasm collection using 13 simple sequence repeat (SSR) markers. A total of 71 alleles were observed across 64 accessions representing 3 Croatian regions (Istria, Dalmatia and continental Croatia) and 16 foreign landraces, with an average of 5.46 alleles per locus. Among the 80 accessions analysed, 61 distinct multilocus genotypes (MLG) were identified, of which 51 represented unique genotypes and the remaining accessions were divided into 10 MLG groups, comprising potential duplicates or redundant genotypes. Model-based Bayesian and hierarchical UPGMA clustering approaches revealed five major groups within the collection which partially correlated with geographical origin. The analysis of molecular variance (AMOVA) showed that the majority (87.71%) of the total molecular diversity is within the Croatian groups of accessions, even though a significant share (12.29%) of diversity derived from genetic diversity among groups. These results support regional structuring, as well as the existence of significant diversity within local populations. This study is the first comprehensive report on an extensive evaluation of genetic resources of garlic maintained by Croatia with the aim of setting the course for future preservation strategies with particular emphasis on the value of diversity in the context of climate change both on macro and micro levels.

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克罗地亚大蒜种质的SSR标记遗传多样性及结构分析

摘要本研究利用13个SSR标记分析了克罗地亚大蒜种质资源的遗传多样性和结构。在克罗地亚3个大区(伊斯特拉、达尔马提亚和克罗地亚大陆)和16个外省的64份材料中共检测到71个等位基因，平均每个位点有5.46个等位基因。在分析的80份材料中，鉴定出61份不同的多位点基因型(MLG)，其中51份代表独特的基因型，其余材料被分为10个多位点基因型组，其中包括潜在的重复或冗余基因型。基于模型的贝叶斯聚类方法和分层UPGMA聚类方法揭示了集合中与地理来源部分相关的五大类群。分子变异分析(AMOVA)表明，大部分(87.71%)的分子多样性来自克罗地亚群体，尽管12.29%的多样性来自群体间的遗传多样性。这些结果支持区域结构，以及当地人口中显著多样性的存在。这项研究是对克罗地亚保存的大蒜遗传资源进行广泛评估的第一份综合报告，其目的是为未来的保存策略制定路线，特别强调在宏观和微观层面上气候变化背景下多样性的价值。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Folia Horticulturae Agricultural and Biological Sciences-Horticulture

CiteScore

3.40

自引率

0.00%

发文量

审稿时长

16 weeks

期刊介绍： Folia Horticulturae is an international, scientific journal published in English. It covers a broad research spectrum of aspects related to horticultural science that are of interest to a wide scientific community and have an impact on progress in both basic and applied research carried out with the use of horticultural crops and their products. The journal’s aim is to disseminate recent findings and serve as a forum for presenting views as well as for discussing important problems and prospects of modern horticulture, particularly in relation to sustainable production of high yield and quality of horticultural products, including their impact on human health.